Agilent’s RNA Spike-In Kit allows researchers to efficiently monitor microarray workflow for linearity, sensitivity and accuracy. Each spike mix contains ten in vitro synthesized, polyadenylated transcripts in predetermined ratios. When they are hybridized onto Agilent microarray control probes, the data needed to track performance and assure confidence is readily accessible.
Agilent’s proprietary Stabilization and Drying Solution is optimized to reduce degradation of cyanine 3 and 5 by ozone during microarray processing in preparation for scanning. It should be used when the ozone level in the microarray processing area is higher than 10 ppb.
The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Universal Human miRNA Reference RNA may also be used as an optimization or standardization reagent for these or other applications aimed at human miRNA analysis.
High-quality total RNA for human, mouse, and rat microarray gene-expression profiling that acts as a consistent control for standard data set comparisons.
Agilent Mass Profiler Professional (MPP) software is a powerful chemometrics platform designed to exploit the high information content of MS data and can be used in any MS-based differential analysis to determine relationships among two or more sample groups and variables.
The Agilent MassHunter METLIN Metabolomics Database and Library enable streamlined metabolite identification to facilitate understanding of global metabolic changes that can occur in a biological system. Available as a comprehensive database of metabolites (PCD) that optionally includes MS/MS spectra (PCDL).
The Agilent Protein 80 and Protein 230 kits provide a reliable way to assess protein concentration, identity, and purity. It eliminates handling of SDS-PAGE slab gels, staining or imaging steps.
The Agilent DNA Fish ID solution uses advanced software to achieve more accurate results – quickly and easily. Key to the process is the Agilent 2100 Bioanalyzer and RFLP Decoder Software performs RFLP pattern matching, which eliminates the need for manual calling
The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.
The VariFlex protein expression system offers vectors with unique tags that enhance the utility of E. coli as an expression host. Included in our tag offering are three different SET tags which increase protein solubility and the SBP purification tag for high-yield, pure proteins.
Allows in vivo excision of cloned fragments in the phagemid vector Screening with both antibodies and nucleic acid probes Each premade library comes with necessary strains and helper phage
This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.
Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.
The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector. In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed. In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…
The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.
The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.
Method completed in less than one day versus 2 days with restriction enzymes
The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).
StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.
The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.
Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.
Restriction Enzymes Dpn I is an ideal enzyme for cloning and Southern blot analysis High purity enzyme preparation Includes 10X universal reaction buffer Glogos II Autorad Markers Luminescent peel-off stickers used for accurately marking and aligning autoradiographs. Easily affixed to plastic wrap or paper, these convenient markers emit low-level luminescence rather than radioactivity, making the Glogos II m…
