Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.
The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.
Our SideStep lysis and Stabilization buffer ensures accurate gene expression studies by eliminating sample loss and degradation. Our proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Following the 10-minute room temperature lysis, you can go directly from cells to QPCR amplification without stopping to purify.
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.
Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible features. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.
The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.
Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems. When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element
Determine the direct or indirect involvement of new gene products, growth factors, and drug candidates in specific pathways with our PathDetect Trans-Reporting Systems. Easily investigate whether your protein or compound influences the activation of any of the six transcription factors offered. These systems are available as plasmid vectors with a luciferase reporter for co-transfection with your gene of interest into the c…
The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.
Hygromycin B is an antibiotic that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…
The MBS Mammalian Transfection kit can increase your transfection efficiencies up to 100-fold. Cells are incubated with CaPO 4 /DNA coprecipitate for only 3 hours, versus 6 to 12 hours with traditional CaPO 4 methods. Additionally, cells can be assayed 12 to 24 hours post-transfection instead of the usual 48 to 72 hours.
The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.
The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.
The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind ® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution.
The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.
The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae. Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.
Bond Elut 96 Square-well Plates are specially designed to offer superior cleanliness, flow reproducibility, and reliability, ensuring trouble-free, high throughput operation. Conversion of cartridge-based methods to an automation-friendly 96-well plate format has never been easier or faster.
Process up to 20 Bond Elut Cartridges at the Same Time Engineered to increase laboratory productivity, the corrosion-resistant Vac Elut 20 allows simultaneous processing of up to 20 Bond Elut cartridges. The manifolds clear glass base allows careful monitoring of the entire sample collection process. Its compact, linear design requires very little bench space.
For Larger Elution Volumes The Vac Elut 20 with a large glass basin and collection rack accommodates larger 16 x 150 mm test tubes. The same high quality material and features on the standard Vac Elut system are incorporated on this special unit. These collection vessels can be utilized in combinatorial chemistry applications using large boiling tubes for collection of purified synthesis mixtures, or for any SPE extractio…
Process up to 24 SPE Cartridges at the Same Time The Vac Elut SPS 24 allows you to process up to 24 SPE cartridges simultaneously. Like all Vac Elut manifolds, the SPS 24 is made from durable, solvent-resistant materials and engineered to last. The glass sides allow easy viewing of the entire sample collection process.
Manifolds and Accessories Agilent manifolds and accessories complement the quality of SampliQ sorbents. Flexible configurations and availability as complete assemblies or individual components mean that the user has the necessary capability at any stage from method development to high-throughput operation. A full selection of adapters allows stacking cartridges for multi-step separation of or use of flexible cartridges on 12…
GC/MS Test and Performance Samples Agilent J&W GC Column Test StandardsLC and LC/MS Standards GPC/SEC and GFC Calibration Standards Amino Acids Standards and Reagents CE Standards and Reagents ICP-MS Standards UV-Vis Standards and Reagents Application Specific Standards Residual Solvent Standards SampliQ QuEChERS Standards GC Instrument Evaluation Analyzer Kit Standards GC Instrument Installation and Calibration Standards
The ion source operates by electron ionization (EI) or chemical ionization (CI). The sample enters the ion source from the GC/MSD interface. Electrons emitted by a filament enter the ionization chamber, guided by a magnetic field. The high-energy electrons interact with the sample molecules, ionizing and fragmenting them. The positive voltage on the repeller pushes the positive ions into the lens stack, where they pass throug…
Agilent has discontinued the line of Cary 600 series infrared microscopy (Cary 610-FTIR) and chemical imaging systems (Cary 620-FTIR). This includes the Cary 610 and Cary 620 Microscopes and Cary 660 and Cary 670 spectrometers and relevant accessories.
