Our miRNA qRT-PCR detection system enables accurate and real-time PCR quantification for miRNA. The kit is a high-specificity reagent system that delivers sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation reaction and then reverse transcribes the polyadenylated RNA into QPCR-ready cDNA.
Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.
The Agilent SideStep II Lysis and Stabilization Buffer uses a single-tube, solution-based format, rather than a column-based format. The patent-pending solution lyses cells in minutes and releases both RNA and DNA molecules into the solution. This immediately stabilizes nucleic acids without further processing required.
The SideStep mRNA Enrichment Kit offers fast and efficient delivery of mRNA directly from cells without purifying total RNA. Save time and money with intuitive 30-minute methods for research applications.
Our SideStep II QRT-PCR products deliver efficient cell lysis and sensitive quantitative gene expression analysis without RNA purification. Utilizing Agilent’s Brilliant QRT-PCR technology, multiple samples can be screened quickly and easily. The one-step process results in RNA stabilized for up to 6 months.
Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.
StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.
StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.
The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures. Robust, cost-effective alternative to Taq Equal to better yield with faster cycling conditions Amplify up to 6 kb genomic DNA targets Easily substituted…
The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.
The Agilent Oligo aCGH Prehybridization Buffer is used to wet and condition arrays for hybridization on a Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. It provides for better distribution of the sample over the active area, and helps prevent the occurrence of gradients in the signals.
The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD). Detects protein-prote…
The InterPlay TAP Purification Buffer Kit is designed to work with the InterPlay Mammalian TAP System and InterPlay Adenoviral TAP System to recover interacting proteins from mammalian cells. The InterPlay TAP Purification Buffer Kit includes the buffers required to perform tandem affinity purification.
Agilent's GeneSpring provides powerful, accessible statistical tools for intuitive data analysis and visualization.
SureCall is a research desktop application combining both novel and widely accepted opensource algorithms for end-to-end NGS data analysis from alignment to categorization and annotation of mutations, supporting Agilent Target Enrichment applications.
Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.
Perform high sensitivity protein electrophoresis with the 2100 Bioanalyzer System. The High Sensitivity Protein 250 assay provides a broad dynamic range with sensitivity down to Silver Stain comparable levels. Efficiency is enhanced by eliminating handling of SDS-PAGE slab gels, staining or, imaging steps.
The Supercoiled DNA Ladder contains fragments from 2 kb to 10 kb at 1-kb increments and an additional band at 12 kb. Each supercoiled band is at a known concentration, with the 7-kb band at a higher concentration than the others. The resulting brighter band in the middle of the ladder acts as a useful reference point. Accurate size determination facilitated by evenly spaced bands Fragments are exact multiples of 1000 bp…
Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The Just cDNA Double-Stranded cDNA Synthesis Kit generates high-quality, blunt-ended cDNA, perfect for use in non-directional cDNA libraries. Makes blunt double-stranded cDNA Can be used for in vitro transcription Perfect for nondirectional cDNA libraries
QuikHyb hybridization solution was developed for use in Southern, Northern, slot-blot and plaque-lift formats using randomly labeled radioactive and nonradioactive nucleic acid probes. Reduces hybridization time from customary 12-24 hours to 1-2 hours Efficient and sensitive radioactive and nonradioactive detection of nucleic acids Doubles signal intensity
Spare parts and accessories available for the Agilent 2100 Bioanalyzer. Instrument troubleshooting & diagnostics for cell assays. Includes cell autofocus chip and documentation.
Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.
