The High-Sensitivity beta-Galactosidase Assay Kit provides an easy, rapid, and sensitive method for determining the beta-galactosidase activity in the lysates of cells transfected with a beta-galactosidase expression construct. Rapid, simple and sensitive >= 10-fold increase in sensitivity over ONPG-based kits Reduces amount of cell extract needed for assay Useful for cells that are difficult to transfect and have l…
Spare parts and accessories available for the Agilent 2100 Bioanalyzer. Instrument troubleshooting & diagnostics for cell assays. Includes cell autofocus chip and documentation.
The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene. Tightly controlled T7 RNA polymerase-based gene expression in E.coli High-level expression upon induction Convenien…
The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene. Tightly controlled T7 RNA polymerase-based gene expression in E.coli High-level expression upon induction Convenien…
The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.
Agilent’s proprietary Stabilization and Drying Solution is optimized to reduce degradation of cyanine 3 and 5 by ozone during microarray processing in preparation for scanning.
The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression. The establishment of a highly regulated expression system in eukaryotic cells is an invaluable tool for the study of cell cycle, oncogenicity, cytogenicity, and the mechanisms of gene regulation.
Randomly pre-sheared DNA Molecular weight ranges from 300 bp to 2000 bp Guaranteed DNase-free
Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.
G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. G418 Sulfate is available as a crystalline solid or in aqueous sterile solution.
MVP RNA is application-ready RNA available in small economical pack sizes. Extensive and rigorous quality control including many application specific assays provides assurance that the RNA is intact, full-length and nearly DNA-free. Extensive quality control ensures high-quality RNA Eliminates tedious, time consuming RNA isolation procedures Application ready for real time RT-PCR, miRNA detection and Northern blot anal…
RNace-it ribonuclease cocktail is a mixture of two RNases: RNase A and a nonspecific RNase from Aspergillus oryzae. The combined action of these ribonucleases results in the degradation of RNA into small oligoribonucleotides. Mixture of RNase A and RNase T1 Combined action of these RNases degrades RNA into small oligoribonucleotides Tested to be free of contaminating DNase activity
The Affinity protein expression and purification system provides a simple, gentle, and effective method for high-level protein expression and purification. A complete Affinity protein expression and purification system includes calmodulin (calcium-modulated protein or CaM) affinity resin and pCAL expression vectors, containing a calmodulin-binding peptide (CBP) tag.
Agilent’s mouse CpG microarrays enable a comprehensive insight into the DNA methylation changes that may be correlated to key biological processes. Such processes include cancer, development, genomic imprinting, gene silencing, and chromatin stability.
Choose the Klenow Fill-In Kit to perform both partial fill-in reactions to prepare genomic DNA for insertion into the Lambda FIX II vector, and complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation. Or, use the kit to radioactively end label a 5 overhang with [alpha- 32 P]dNTP.
The Luciferase Assay Kit provides rapid, sensitive, and quantitative measurement of American firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. Sensitive, widely used reporter assay Rapid, quantitative results Reporter activity immediately available upon translation Useful in conjunction with PathDetect systems
The Mammalian Two-Hybrid Assay Kit is a powerful method for detecting protein-protein interactions in vivo in mammalian cells. The two-hybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression. Detects protein-protein interactions in mammalian cells Works well in a variety of mammalian cell lines Confirms suspected interactions between two proteins or i…
The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.
The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments. The Agilent miRNA Kit may also be used as an optimization or standardization reagent for human miRNA analysis. Available in industrial lots for high throughout applications.
Agilent Mouse microRNA Microarrays generate complete microRNA profiles for broad insight into mouse expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands. Content regularly updated from the Sanger miRBase 16.0 Mouse and mouse viral miRNAs represented Optimal sensitivity and specificity through unique pro…
Agilent Mouse microRNA Microarrays generate complete microRNA profiles for broad insight into mouse expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands. Content regularly updated from the Sanger miRBase 16.0 Mouse and mouse viral miRNAs represented Optimal sensitivity and specificity through unique pro…
Agilent Mouse microRNA Microarrays generate complete microRNA profiles for broad insight into mouse expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands. Content regularly updated from the Sanger miRBase 16.0 Mouse and mouse viral miRNAs represented Optimal sensitivity and specificity through unique pro…
Agilent Mouse microRNA Microarrays generate complete microRNA profiles for broad insight into mouse expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands. Content regularly updated from the Sanger miRBase 16.0 Mouse and mouse viral miRNAs represented Optimal sensitivity and specificity through unique pro…
Agilent Mouse microRNA Microarrays generate complete microRNA profiles for broad insight into mouse expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands. Content regularly updated from the Sanger miRBase 16.0 Mouse and mouse viral miRNAs represented Optimal sensitivity and specificity through unique pro…
