Separations is a fundamental scientific discipline focused on the isolation and purification of components from mixtures. Through techniques such as chromatography, distillation, and filtration, separations scientists separate substances based on their physical or chemical properties. This field plays a crucial role in various industries, including pharmaceuticals, food and beverage testing, environmental science, materials research, and more. Explore how separations techniques enable precise analysis, quality control, and the development of innovative products.
Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive of all alkyl group bonded phases for nonpolar compounds. However, due to the extensive endcapping of this sorbent to mask polar silanol activity, retention, and elution of polar and multifunctional analytes can still be achieved.
Bond Elut EWP is based upon standard particles size silica but with 500 pores to allow more efficient extraction of large molecules (>15,000 MW), which are typically excluded from standard porosity silica phases.
Bond Elut C18 OH is a nonendcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
Bond Elut C2 is a fairly nonpolar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.
Bond Elut C8 is very similar in property to C18, but is not as retentive for nonpolar compounds due to its shorter hydrocarbon chain and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution.
Bond Elut Carbon cartridges are packed with ultrapure graphitized carbon particles that have been optimized for the absorption of pigments in food, fruits and vegetables, and small organic residues in wastewater. The powerful retention mechanisms of these products are appropriate for a broad range of analytes. In addition, careful manufacturing techniques result in lower carbon fines on the wall of the device.
CBA is a midpolarity sorbent and weak cation exchanger (pKa 4.8). It can be used with a wider range of counterions than lower pKa sorbents like SCX, and will demonstrate easier elution of quaternary amine functionalized analytes.
Bond Elut Cellulose columns utilize a pure microgranular cellulose powder that is packed between two, 20 µm polyethylene frits. The cellulose phase is very stable over a wide pH range and contains an extremely low metal content (Fe and CU content less than 5 ppm).
The Bond Elut Certify extraction cartridge utilizes a packed bed consisting of a special, nonpolar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extracting a wide range of compounds from a diverse number of aqueous matrices.
Bond Elut Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and strong anion-exchange (SAX) sorbent. It has been optimized for acidic drugs, such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen, and other compounds tha…
