Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.
The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems.
The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems.
The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems.
Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…
These competent cells offer the highest transformation efficiencies available, enabling dramatically improved transformation efficiencies when cloning large plasmids and ligated DNA.
This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.
This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.
Agilent offers the SCS110 and JM110 strains, which lack both dam and dcm activity. DNA propagated in these strains can be digested by methylation-sensitive enzymes such as Xba I, Cla I and EcoR II. SCS110 is an endA– derivative of the JM110 strain.
