Isopropyl-β-D-thiogalactoside (IPTG) is used in combination with X-gal to detect β-galactosidase activity in blue/white selection assays. When the appropriate vectors are used, blue white screening is an important tool for screening and selecting relevant colonies.
Ampicillin, IPTG, and X-Gal in convenient packaging. G418 Sulfate Hybromycin B
Agilent X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, BCIG) is a premium quality chromogenic substrate of β-Galactosidase. It is used with cloning systems that utilize β-galactosidase activity as an indicator of successful cloning.
The Agilent miRNA Spike-in kit allows researchers to monitor the miRNA labeling and hybridization reactions. The Spike-in kit contains a labeling and hybridization spike-in, which correspond to complementary probe sequences on the microarrays. The Spike-ins and probes are designed using Drosophila.
The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures. Robust, cost-effective alternative to Taq Equal to better yield with faster cycling conditions Amplify up to 6 kb genomic DNA targets Easily substituted…
The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. New cDNA synt…
The StrataCooler LP Benchtop Cooler has a capacity of 32 1.5 mL tubes and weighs 1.2 kg. The StrataCooler LP Benchtop Cooler provides the most protection for your enzymes in the freezer or on the benchtop. It maintains -15 for at least 2 hours.
The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay. More sensitive than traditional assays (use as few as 10e4 cells) Enhancer is non-cytotoxic and non-denaturing Up to 40-fold signal enhancement for cultured cells Proprietary enhancer which increases signal at lea…
The Mitochondrial membrane potential detection kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential. In healthy non-apoptotic cells, the dye stains the mitochondria bright red. Detect the loss in mitochondrial membrane potential - Early indicator of cellular apoptosis Convenient kit format Use with flow cytometer, fluorescence plate reader, fluorescence microscope
