Our SideStep II QRT-PCR products deliver efficient cell lysis and sensitive quantitative gene expression analysis without RNA purification. Utilizing Agilent’s Brilliant QRT-PCR technology, multiple samples can be screened quickly and easily. The one-step process results in RNA stabilized for up to 6 months.
Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.
StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.
StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.
Agilent’s RNA spike-In kit lets researchers efficiently monitor their microarray workflow for linearity, sensitivity, and accuracy. Each spike mix in these one-color and two-color RNA spike-in kits contains 10 in vitro synthesized, polyadenylated transcripts in predetermined ratios.
Agilent’s RNA spike-In kit lets researchers efficiently monitor their microarray workflow for linearity, sensitivity, and accuracy. Each spike mix in these one-color and two-color RNA spike-in kits contains 10 in vitro synthesized, polyadenylated transcripts in predetermined ratios.
Agilent's Ampicillin tabs provide a high quality tablet option to select for ampicillin-resistant bacteria during analysis. The Ampicillin ready made solution is for research use only and provided in powder form. The tablets correct for 6-aminopenicillanic acid, 6-APA, and are used to reduce satellite colonies when selecting for ampicillin-resistant bacteria. Turbo Amp Antibiotic is used to ensure precise results.
Isopropyl-β-D-thiogalactoside (IPTG) is used in combination with X-gal to detect β-galactosidase activity in blue/white selection assays. When the appropriate vectors are used, blue white screening is an important tool for screening and selecting relevant colonies.
Ampicillin, IPTG, and X-Gal in convenient packaging. G418 Sulfate Hybromycin B
Agilent X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, BCIG) is a premium quality chromogenic substrate of β-Galactosidase. It is used with cloning systems that utilize β-galactosidase activity as an indicator of successful cloning.
The Agilent miRNA Spike-in kit allows researchers to monitor the miRNA labeling and hybridization reactions. The Spike-in kit contains a labeling and hybridization spike-in, which correspond to complementary probe sequences on the microarrays. The Spike-ins and probes are designed using Drosophila.
The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures. Robust, cost-effective alternative to Taq Equal to better yield with faster cycling conditions Amplify up to 6 kb genomic DNA targets Easily substituted…
The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. New cDNA synt…
The StrataCooler LP Benchtop Cooler has a capacity of 32 1.5 mL tubes and weighs 1.2 kg. The StrataCooler LP Benchtop Cooler provides the most protection for your enzymes in the freezer or on the benchtop. It maintains -15 for at least 2 hours.
The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay. More sensitive than traditional assays (use as few as 10e4 cells) Enhancer is non-cytotoxic and non-denaturing Up to 40-fold signal enhancement for cultured cells Proprietary enhancer which increases signal at lea…
The Mitochondrial membrane potential detection kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential. In healthy non-apoptotic cells, the dye stains the mitochondria bright red. Detect the loss in mitochondrial membrane potential - Early indicator of cellular apoptosis Convenient kit format Use with flow cytometer, fluorescence plate reader, fluorescence microscope
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. CMV promoter for high-level protein expression in mammalian cells Large multiple cloning site contains 15 unique sites Clone PCR products generated with any PCR e…
The Agilent Oligo aCGH Prehybridization Buffer is used to wet and condition arrays for hybridization on a Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. It provides for better distribution of the sample over the active area, and helps prevent the occurrence of gradients in the signals.
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.
The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD). Detects protein-prote…
The InterPlay TAP Purification Buffer Kit is designed to work with the InterPlay Mammalian TAP System and InterPlay Adenoviral TAP System to recover interacting proteins from mammalian cells. The InterPlay TAP Purification Buffer Kit includes the buffers required to perform tandem affinity purification.
Agilent's GeneSpring provides powerful, accessible statistical tools for intuitive data analysis and visualization.
SureCall is a research desktop application combining both novel and widely accepted opensource algorithms for end-to-end NGS data analysis from alignment to categorization and annotation of mutations, supporting Agilent Target Enrichment applications.
