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AccuPrep® 96 PCR Purification Kit for BioVac 96 Vacuum Manifold, 96-well plate x 2; Refer to K-3034 AccuPrep PCR Purification Kit

Bioneer

Bioneer’s AccuPrep® 96 PCR Purification Kit is a high throughput PCR purification kit that allows PCR product purification from up to 96 PCR reactions at once. The AccuPrep 96 PCR Purification Kit allows purification of up 10 μg of DNA fragments from PCR and other enzymatic products within 25 minutes. The AccuPrep 96 PCR Purification Kit features recovery efficiencies up to 95%. Size range is 100 bp - 10 kb, thus 20-40 mer oli…

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Tissue RNA PrepMate™; Tissues, serum, leukocytes, cultured cells, 100 extractions

Bioneer

The Tissue RNA PreMate™ kit is designed for total RNA extraction from tissue, cultured cells, leukocytes or serum. The PreMate protocol is based on a Modified guanidinium salt-lysis method to optimally extract total RNA. Extracted total RNA is treated with PreMate’s RNase-free DNase to eliminate any genomic DNA. The RNA Isolated with the Tissue RNA PreMate kit is suitable for Northern analysis, dot blot hybridization, poly A s…

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Viral RNA PrepMate™; RNA virus (HCV), tissues, serum, cultured cells, leukocytes, 100 extractions

Bioneer

Viral RNA PreMate contains all of the required reagents for easy isolation of total RNA from RNA viruses such as HCV using the guanidinium salt-lysis method. Possible sources include tissue, cultured cells, leukocytes, and serum. The extracted RNA can be used for molecular cloning, RT-PCR, Northern analysis, dot blot hybridization, poly-A (+) selection, RNase protection assays, and other application that require pure RNA. Vira…

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DNA PrepMate™ - II; Bacteria, PCR product, agarose gel fragment, enzyme reation mixtures, 150 extractions

Bioneer

DNA PrepMate™ II uses an optimal binding buffer and silica matrix to effectively isolate DNA from bacterial cells, PCR products, gel fragments and enzymatic reaction mixtures. Examples include the purification of DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gel, high salt solutions, and lysed bacterial cells (using alkaline lysis). DNA preparation is accomplished quickly and efficiently. The size range fo…

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DNA Phosphoramidites

Bioneer

Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…

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RNA Phosphoramidites

Bioneer

Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…

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Amino Linker Phosphoramidites

Bioneer

Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…

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Spacer Phosphoramidite

Bioneer

Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…

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