Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…
Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…
Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…
Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…
Phosphoramidites for nucleic acid labeling, microarray labeling, and moreFeatures• Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.• t-butylamine/H2O/MeOH is recommended for cleavage and deprotection steps of TAMRA modified oligos.• dI-phosphoramidite can perform as an universal base as it hybridizes with any of the four bases.Technical Info…
High load CPG prepackaged Universal solid support for DNA SynthesisControlled Pore Glass (CPG) is the gold standard for solid phase oligonucleotide synthesis, with features such as high surface area, tightly controlled pore size and chemical inertness.Our High load CPG for DNA synthesis is available with or without phosphoramidites and can be purchased for standard synthesis sizes or for long-mer synthesis.Features• Utilizes C…
dNTP's from Bioneer are cost effective and hightly pure. Bioneer is one of the few manufacturers that actually produces our own phosphoramidites. Our dNTP's are made at our state-of-the-art manufacturing facilities, and Bioneer controls quality every step of the way in order to provide you with the best value for your research dollar.
25/100 bp Mixed DNA Ladder is designed for determining the size of double stranded DNA from 25 to 2,000 bp. The ladder consists of 17 double stranded DNA fragments ranging in size from 25 to 200 bp in 25 bp increments, from 200 to 1,000 bp in 100 bp increments, and an additional fragment of 2,000 bp. The DNA marker fragments at 150, 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
25/100 bp Mixed DNA Ladder is designed for determining the size of double stranded DNA from 25 to 2,000 bp. The ladder consists of 17 double stranded DNA fragments ranging in size from 25 to 200 bp in 25 bp increments, from 200 to 1,000 bp in 100 bp increments, and an additional fragment of 2,000 bp. The DNA marker fragments at 150, 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
100 bp DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 2,000 bp. The 100 bp DNA Ladder consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
100 bp DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 2,000 bp. The 100 bp DNA Ladder consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
100 bp Plus DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 10,200 bp. The 100 bp Plus DNA Ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp. The 200, 1,000 and 2,000 bp bands are two times brighter for easy identificati…
100 bp Plus DNA Ladder was designed to determine the size of double stranded DNA fragments from 100 to 10,200 bp. The 100 bp Plus DNA Ladder consists of 19 double stranded molecular weight markers, ranging in size from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000, 2,961, 4,000, 5,007, 5,991, 8,000, 10,200 bp. The 200, 1,000 and 2,000 bp bands are two times brighter for easy identificati…
1 kb DNA Ladder is designed for determining the size of double stranded DNA ranging between 500 to 10,000 bp. The 1 kb Ladder consists of DNA fragments 0.5, 1.0, 1.6, 2.0, 3 (2.961), 4, 5 (5.007), 6 (5.991), 8 and 10 (10.2) kb. The 2.961 kb band is approximately two times brighter for easy identification.
1 kb DNA Ladder is designed for determining the size of double stranded DNA ranging between 500 to 10,000 bp. The 1 kb Ladder consists of DNA fragments 0.5, 1.0, 1.6, 2.0, 3 (2.961), 4, 5 (5.007), 6 (5.991), 8 and 10 (10.2) kb. The 2.961 kb band is approximately two times brighter for easy identification.
Lambda DNA/EcoR I Marker is created by digesting lambda DNA with the restriction enzyme EcoR I. The resulting DNA ladder is then purified and buffer and dye are added so the DNA marker is ready to load. Lambda DNA/EcoR I Marker consist of 6 double stranded lambda DNA fragments ranging in size from 3,530 to 21,226 bp.
Lambda DNA/EcoR I Marker is created by digesting lambda DNA with the restriction enzyme EcoR I. The resulting DNA ladder is then purified and buffer and dye are added so the DNA marker is ready to load. Lambda DNA/EcoR I Marker consist of 6 double stranded lambda DNA fragments ranging in size from 3,530 to 21,226 bp.
Lambda DNA/Hind III DNA Marker is generated by digesting lambda DNA with restriction enzyme Hind III. The resulting DNA Ladder is then purified, and buffer and dye are added so the marker is ready to load. Lambda DNA/Hind III Marker consist of 8 double stranded Lambda DNA fragments ranging in size from 125 to 21,226 bp, making it an ideal molecular weight marker for daily use.
Lambda DNA/Hind III DNA Marker is generated by digesting lambda DNA with restriction enzyme Hind III. The resulting DNA Ladder is then purified, and buffer and dye are added so the marker is ready to load. Lambda DNA/Hind III Marker consist of 8 double stranded Lambda DNA fragments ranging in size from 125 to 21,226 bp, making it an ideal molecular weight marker for daily use.
Lambda DNA/EcoR I + Hind III DNA Marker is generated by digesting lambda DNA with two restriction enzymes EcoR I and Hind III. The resulting DNA Ladder is then purified and buffer and dye are added so the marker is ready to load. Lambda DNA/EcoR I + Hind III Marker consist of 13 double stranded Lambda DNA fragments ranging in size from 125 to 21,226 bp, making it an ideal molecular weight marker for daily use.
Lambda DNA/EcoR I + Hind III DNA Marker is generated by digesting lambda DNA with two restriction enzymes EcoR I and Hind III. The resulting DNA Ladder is then purified and buffer and dye are added so the marker is ready to load. Lambda DNA/EcoR I + Hind III Marker consist of 13 double stranded Lambda DNA fragments ranging in size from 125 to 21,226 bp, making it an ideal molecular weight marker for daily use.
For routine applications, Bioneer offers standard custom oligos with no modification, Bio-RP purification, provided in a tube format. The chart in the ordering information tab shows pricing at various synthesis scales, but please contact your sales representative to learn about any institutional or volume discounts we might have in place for our oligonucleotides.
A total capacity of > 30,000 oligonucleotides per day gives Bioneer the opportunity to address the needs of researchers that require very large numbers of oligonucleotides. In-house production of raw materials along with proprietary high throughput oligo synthesis and purification systems result in much higher quality oligonucleotides at significantly lower cost. Bioneer understands that high quality and low cost is especially…
Bioneer provides the ideal tools for your specialized applications. With the advent of technologies such as real-time detection and quantification, as well as mass-scale genotyping projects, more and more scientists require the use of modified oligos. All common Oligo modifications are available. Our fluorogenic oligos for qPCR, are unsurpassed in quality, and priced for value. In addition to the modifications listed in our or…
Extendamers™ are oligonucleotides from 130 - 200 bases. Bioneer specializes in long oligo synthesis and provides them with Bio-RP or PAGE purification. As with all Bioneer Oligonucleotides, Extendamers are QC tested by MALDI-TOF after oligo synthesis, ensuring the highest quality products for your research needs. Bio-RP (or PAGE) purification also ensures you have only full length oligonucleotides for your experiments. Use Ext…
