A simple and straightforward procedure for measuring functional kinase activity.The Eu(K) labeled antibody and XL665 conjugate are added simultaneously after the enzymatic step. Results are available after a 2-hour incubation at room temperature. The two reagents are stable in solution, even in an excess of EDTA (0.5M), while the assay remains stable for >24 hours. An extended list of Eu3+-Cryptate labeled phospho-specific…
An homogeneous fluorescence assay for PGE2Prostaglandin E2 (PGE2) is a primary product of arachidonic metabolism in many cells. Under inflammatory stimuli, PGE2 is de novo synthesized by three enzymatic steps via the cyclooxygenase (COX) pathways. PGE2 production is commonly used in the detection of COX-1 and COX-2 activity. In the PGE2 HTRF® assay, free PGE2 from the sample competes with XL665 labeled PGE2 for binding to a Cr…
Streamlined single plate protocols including all stimulation and cytokine quantification.The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynamics, and no use of isotopes. Procedures are performed on a single plate with simultaneous cell stimulation and cytokine detection. They can all be run under a similar experimental procedure stream…
A homogeneous single plate assay for the sensitive quantification of histamine.Histamine is a small biogenic amine that plays an important role in several physiological and pathophysiological processes. The histamine HTRF® assay is based on the competition between sample histamine and XL665-labeled histamine for binding to a Cryptate-labeled antibody. The amount of histamine detected is inversely proportional to the specific f…
A streamlined assay for human monoclonal antibody screening.The human Fc check kit is a competitive immunoassay involving a Cryptate conjugated polyclonal antibody specific to human Fc IgGs and XL665 coupled human IgGs. This homogeneous assay enables a precise assessment of all human IgG isotypes within 3 hours at room temperature and is particularly adapted to human monoclonal antibody (MAb) screening. Like all other HTRF® as…
A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stab…
A unique set of reagents for the investigation of ubiquitin.The HTRF® Ubiquitin toolbox assembles all the reagents for flexible assay development and HTS of each step of the ubiquitin system. Ubiquitin is directly labeled with the Eu3+-Cryptate. This conjugate is directly incorporated into the ubiquitin chain during the enzymatic step. Upon the addition of the XL665 conjugate, the HTRF® signal is proportional to the level of u…
A team of experts for outsourcing your assay developmentsOur Service Team at Cisbio international is highly trained in protein chemistry and fluorescent technology, and offers you its expertise in assay development to build HTRF® assays adapted to your specific targets or to convert existing assays to HTRF® technology.Assays may be developed using HTRF® toolbox reagents, custom labeled molecules or a combination of both. Most…
A fast and reliable process for biomolecule labelingIn many circumstances, assay development and HTS involve specific labeling of the various assay components. Cisbio international’s labeling service is an excellent alternative for the labeling of proteins, peptides, oligonucleotides, or other biomolecules either with Cryptate, XL665, biotin or DNP when you do not have the time or the facilities to label them yourself.Two type…
EPIgeneous™ Binding Domain Assay Kits for studying epigenetic readers. This biochemical assay platform enables a no-wash, sensitive, reliable detection of protein/protein interaction between epigenetic binding domains and histone sequences modified by epigenetic enzymes. Three assay kits (A, B and C) have already been validated and fully optimized on a selection of 28 key binding domains. For non-validated reader domains, a f…
