Corning® Collagen I, human, 0.25mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. (Same as Cat. #40243)SourceHuman PlacentaQualityElectrophoretically homogeneous (SDS-PAGE)Shown to promote attachment and spreading of HT-1080 human fibrosarcoma cellsTested and found negative for bacteria, fungi,…
Corning® Collagen IV, human, 0.25mg, is used as a thin coating on tissue-culture surfaces to promote cell attachment and proliferation and to study the collagen's effects on cell behavior.SourceHuman placenta. NOTE: Source material tested for HBsAG, for anti-HIV-1 and anti-HIV-2, and anti-HCV virus-1 and virus-2.QualityElectrophoretically homogeneous (SDS-PAGE)Tested and found negative for bacteria, fungi, and mycoplasmaPrepar…
Corning® PuraMatrix™ Peptide Hydrogel is a synthetic matrix used to create defined three dimensional (3D) micro-environments for a variety of cell culture experiments. To achieve optimal cell growth and differentiation, it is necessary to determine the appropriate mixture of this material and bioactive molecules (e.g., growth factors, extracellular matrix (ECM) proteins, and/or other molecules). Corning PuraMatrix Peptide Hydr…
Corning® Cell Recovery Solution, 100 mL, enables the recovery of cells cultured on Corning® Matrigel® Basement Membrane Matrix for subsequent biochemical analyses.QualityTested for ability to depolymerize a 1mm thick layer of gelled Matrigel Matrix after one hour at 2° to 8°CFiltered (0.2 µm membrane) and tested for bacteria, fungi, and mycoplasmaPreparation and StorageStable for at least three months at 2° to 8°C.HandlingCorn…
These collagens have been used to culture a variety of cell types to promote adhesion, growth, and/or differentiation. Source Bovine Articular Cartilage Quality Bovine Collagen Type II is a membrane filtered (0.2um) preparation. >95% by SDS-PAGE Found negative for bacteria and fungi. Preparation and Storage Stable for a minimum of 3 months from date of shipment when stored at -70°C. Handling Used for attachment a…
Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.Molecular Weight900 kD (unreduced)SourceEngelbreth-Holm-Swarm (EHS) mouse tumorQualityPurity >/ 90% by SDS-PAGETested for ability to initiate neurite outgrowth of NG-108 Mouse neuroblastoma cellsFound negative for the pres…
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Preparation and StorageStore at -20°C. Avoid multiple freeze-thaws.SourceEngelbreth-Holm-Swarm Mouse TumorQualit…
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Source Engelbreth-Holm-Swarm Mouse Tumor Quality Mouse colonies routinely screened for pathogens via Mous…
Corning® Collagen I, human, 10mg, is effective as a gel or thin coating at a recommended concentration of 0.2-2µg/cm2 of growth surface depending on cell type (guidelines for coating included with product).FormulationFrozen in 2mM HClSourceHuman placenta. Source material tested for hepatitis B antigen and HIV-1 antibody.QualityElectrophoretically homogeneous (SDS-PAGE)Shown to promote attachment and spreading of HT-1080 human…
Corning® ITS Premix Universal Culture Supplement, 5 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 5 mL (5L equivalent), 1/packThe ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin…
