DyNAmo™ SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse transcriptase in the kit is M-MuLV RNase H+, which p…
DyNAmo™ Capillary SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity with instruments using capillary tubes. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse tra…
Finnzymes’ Phire™ Hot Start DNA Polymerase is a novel PCR enzyme. The polymerase incorporates a specific DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, it our unique hot start technology allows complete reactivation of Phire DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymeras…
Finnzymes' DyNAmo™ Flash Probe qPCR Kit allows enhanced performance for probe-based qPCR. Optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillar…
Finnzymes’ novel approach, Direct PCR, saves you time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Finnzymes' Phusion® High-Fidelity DNA Polymerases and Phire® Hot Start DNA Polymerase. These unique fusion enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives them extraordinary properties. Phusion and Phire DNA Polymerases are except…
Finnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…
For amplification of DNA directly from plant material. Finnzymes’ Phire® Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA isolation is required prior to the PCR. The kit is based on Phire® Hot Start DNA Polymerase, a specially engineered enzyme with a unique DNA binding domain. Phire DNA Polymerase is extremely robust and tolerant of many PCR inhibitors present in plant material. The kit incl…
