KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. Features: Reduce cycling times up to 75% Extension times as low as 1 sec per kilobase Broad c…
From tail to type in 1 hour! KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast…
The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the fi…
KAPA Blood DNA Polymerase is a second-generation enzyme derived through a process of directed evolution, and is the first DNA polymerase engineered specifically for the amplification of DNA directly from whole blood. DNA fragments may be amplified directly from reactions containing 1%-20% (v/v) whole human blood without pretreatment of blood samples or DNA isolation, significantly reducing contamination risk, turnaround tim…
KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq i…
High-quality, molecular-grade dNTPs. Kapa offers premium grade dNTPs for PCR. KAPA dNTP Mix contains dATP, dCTP, dGTP, and dTTP supplied at a concentration of 10 mM each. Applications: Standard PCR qPCR cDNA synthesis DNA sequencing DNA labeling Microarrays
High-quality ligase. KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme efficiently joins blunt and cohesive end termini, as well as repairs single-stranded nicks in duplex DNA, RNA, and DNA/RNA hybrids. Applications: Routine subcloning Recircularization of linear DNA Library construction Linker ligation
Fast and efficient ligation. The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designated to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature. KAPA Rapid Ligase contains T4 DNA Ligase, which catalyzes the formation of phosphodiester bond between 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. Features: 5-minute ligati…
