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Nitrotyrosine ELISA

Merck

Upstate's new Nitrotyrosine Assay Kit, Chemiluminescence Detection is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including white high binding 96-well plates, nitrated BSA standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers. The assay has a wide dynamic range and high precision, making…

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cGMP HTS Immunoassay Kit (Chemiluminescent)

Merck

Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Ra…

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phospho-Akt (Thr308) STAR ELISA Kit

Merck

The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The AKT plate is coated with a specific mouse monoclonal anti-AKT capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…

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phospho-Akt (Ser473) STAR ELISA Kit

Merck

The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Akt plate is coated with a specific mouse monoclonal Akt capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incub…

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EGFR STAR ELISA Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal anti-EGFR capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR…

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Phospho-EGFR (Tyr1068) STAR Assay ELISA Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal EGFR capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR antig…

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phospho-Src (Tyr418) STAR ELISA Kit

Merck

The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Src plate is coated with a specific mouse monoclonal anti-Src capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…

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phospho-Met (Tyr1230/Tyr1234/Tyr1235) STAR ELISA Kit

Merck

The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Met plate is coated with a specific mouse monoclonal anti-Met capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…

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PRAS40 STAR ELISA Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The PRAS40 plate is coated with a specific mouse monoclonal PRAS40 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit ar…

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phospho-PRAS40 (Thr246) STAR ELISA Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The PRAS40 plate is coated with a specific mouse monoclonal PRAS40 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit ar…

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STAR FAK ELISA Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The FAK plate is coated with a specific mouse monoclonal FAK capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing FAK antigen…

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Insulin Receptor (β subunit) STAR ELISA Assay Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The IR plate is coated with a specific mouse monoclonal IR (β subunit) capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standards included in the…

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phospho-IR (β Subunit) (Tyr 1162/1163) STAR ELISA Assay Kit

Merck

The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The IR plate is coated with a specific mouse monoclonal IR (β subunit) capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standards included in the k…

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cAMP HTS Immunoassay Kit (Chemiluminescent)

Merck

Upstate®'s cAMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic AMP from cell extracts of any species. The kit comprises a specific anti-cAMP antibody that recognizes all species, an alkaline phosphatase (AP)-labeled cAMP conjugate, ten 96 well microplates pre-coated with an anti-Rabbit antibody, cAMP standard, and an Alkaline Phosphatase chemiluminescence…

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EZ-TFA (Universal Transcription Factor Assay, colorimetric)

Merck

The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity t…

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Universal EZ-TFA Chemiluminescent Transcription Factor Assay

Merck

The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity t…

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ssDNA Apoptosis ELISA Kit

Merck

The CHEMICON ssDNA Apoptosis ELISA Kit is a convenient, sensitive method for early detection of apoptosis. The assay can be performed in two formats: 1. Cells are grown, treated with apoptosis inducing agents, stained and analyzed in the same microtiter plate. This assay is suitable for high throughput screening when induction of apoptosis is used as the endpoint of drug activity. 2. Cell suspensions obtained from cultures or…

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ChemiKine Neurotrophin 3, Sandwich ELISA

Merck

With the ChemiKine assay system, sheep polyclonal antibodies generated against human NT-3 are coated onto a microplate and are used to capture NT-3 from a sample. NT-3 specific, biotin conjugated, mouse monoclonal antibodies detect the captured NT-3. After addition of streptavidin-enzyme, substrate and stop solution the amount of NT-3 is determined. The standard curve demonstrates a direct relationship between Optical Density…

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ChemiKine Nerve Growth Factor, Sandwich ELISA

Merck

With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay…

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ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA

Merck

ChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat. ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay…

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ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA

Merck

ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin. With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects…

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QuantiMatrix Human Fibronectin ELISA

Merck

The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference.

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