Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powe…
The EZ-Zyme™ Chromatin Prep kit allows ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length. Compared to sonication, this procedure not only allows mapping the protein-DNA association at a higher resolution, but also renders the subsequent immunoprecipitation more efficient due to…
Sterivac vacuum-driven filter units are ideal for bottle-top filtering directly into receiver flasks at flow rates as fast as 1.5 L/min. The unique "start & stop" filtration action makes setting up, filtering and transferring the unit to another receiver fast and easy. Push a button to stop filtering, move the device to an empty bottle and restart
• Concentrating and desaltingchromatography column eluates• Concentrating monoclonal antibodies• Concentrating proteins or viruses from culture supernatants• Clarifying tissue homogenates and cell lysates• Buffer exchange or diafiltration
High recovery Durapore (PVDF) and hydrophilic PTFE membranesFive different pore sizes from 0.1 to 5.0 µmPre-sterilized units also availableFast filtration and highly reproducible performanceUse in fixed-angle rotors for 15 mL tubes
Easy, pre-sterilized, centrifugal sample clarification units for either 0.5 mL (MC) or 2 mL (CL) maximum volumes.High recovery Durapore (PVDF) membraneFast filtration and highly reproducible performanceUse in fixed-angle rotors for 1.5 mL tubes (MC) or 15 mL tubes (CL)
Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…
Concentrates 2 mL down to 15-70 µL 98% sample recovery Fast processing time - 10 to 30 minute spin times Engineered dead stop Reverse spin capability provides consistent recoveries
Automation compatible filter units from EMD Millipore are specially designed to provide efficient filtration for dissolution and other automated sample preparation applications.Choice of Filter Media: Automation compatible filter units are available in a variety of membrane types and pore sizes, including units with prefilters for particulate-laden samples.Automation Compatible Packaging: The 200 pack comes preassembled in 8 t…
Millex filter units with hydrophobic Fluoropore membrane are ideal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions. Specialized filter units protect hemodialysis transducers from blood and moisture. 50 mm Millex filter units are also used for vacuum line protection. All units are bi-directional
This 13 mm syringe filter with a Hydrophilic Polyethersulfone membrane, for non-sterile filtration, has a 0.45 micron pore size used in preparation of aqueous & mild organic solutions prior to chromatography or other instrument analyses.
Millex-GP Filter for Samplicity G2, 0.22 µm, PES 33 mm, non-sterile
The CpGenome™ DNA Modification Kit is used to create methylation-specific sequence alterations in any DNA sample. Using chemical treatment, the kit converts all unmethylated cytosine residues to uracil. Methylated cytosine residues are unaltered in this process. Therefore, the chemically-treated DNA sequence will differ only if it was originally unmethylated in vivo. After sequence conversion, methylation specific PCR is emplo…
The CpGenome™ Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.
ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…
Introducing Direct Detect™, the first infrared (IR)-based biomolecular quantitation system. Just spot your sample, blank, and read! No sample prep, standard curves, messy cuvettes or liquid waste. With inherent amide bond detection, the Direct Detect™ system distinguishes proteins and peptides from interfering sample components, providing more accurate results without the pitfalls of colorimetric assays. Technology: FTIR Spe…
Indispensable for NMR spectroscopy Unlocking the molecular structure of organic molecules is central to understanding how they interact with other molecules. This is especially critical in genomics, proteomics and drug discovery research. The Nuclear Magnetic Resonance (NMR) technique allows scientists to “see” molecular structure and dynamics. MagniSolv™ deuterated solvents are a key tool for the NMR technique. MagniSolv™ deu…
The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…
The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…
The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…
The quality of sequence obtained from Next Generation sequencing platforms such as Illumina, Inc. requires the accurate determination of input library DNA. Loading too much DNA can lead to overlapping clusters and loss of read counts whereas too little input can lead to poor read lengths and lower sequence output. The PureGenome™ NGS Library Validator Kit measures the absolute amount of adapter-ligated, sequence ready DNA in I…
Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has o…
Eshmuno® CPX media - High aggregate removal efficiency combined with high dynamic binding capacity Eshmuno® CPX media is a strong cation exchanger built on the proven Eshmuno® resin technology. Eshmuno® CPX media combines high aggregate removal efficiency in downstream purification linked to an outstanding high dynamic binding capacity, utilizing the 50 μm Eshmuno® base bead technology and EMD Millipore’s proprietary tentacle…
