Dextran Sulfate (MW 500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.
Hybrisol® Hybridization Solutions are pre-made, ready-to-use solutions optimized for sensitive single and multiple gene copy detection using membrane-based hybridization techniques. They are designed to give maximum signal and clean background for hybridizations performed on nylon and nitrocellulose membranes. Hybrisol® Solution contains 10% Dextran Sulfate, 1% SDS, 50% Formamide, 6X SSC, Sheared DNA, and Modified Denhardt's S…
The CpG WIZ® p16 Amplification Kit is used for determining the methylation status of the p16 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified such that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated)…
The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unm…
The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and…
The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also i…
The CpGenome™ DNA Modification Kit is used to create methylation-specific sequence alterations in any DNA sample. Using chemical treatment, the kit converts all unmethylated cytosine residues to uracil. Methylated cytosine residues are unaltered in this process. Therefore, the chemically-treated DNA sequence will differ only if it was originally unmethylated in vivo. After sequence conversion, methylation specific PCR is emplo…
CpGenome™ Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.
CpGenome™ Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies.
The CpGenome™ Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.
The CpGenome™ DNA Modification Kit (Cat. No. S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® BRCA1 Amplification Kit contains the reagents required for the PCR amplification reactions.
The MILLIPLEX® MAP Rat Osteocalcin - Single Plex is used for the simultaneous quantification of the following Rat analytes: Osteocalcin
The MILLIPLEX® MAP Rat OPG - Single Plex is used for the simultaneous quantification of the following Rat analytes: Osteoprotegerin
The MILLIPLEX® MAP Rat RANKL Single Plex is used for the simultaneous quantification of the following Rat analytes: RANKL
The MILLIPLEX®MAP Human Brain-Derived Protein Panel is used for the simultaneous quantification of the following Human analytes: ACTH, AGRP, BDNF, CNTF, FSH, GH, LH, Prolactin, TSH
The MILLIPLEX® MAP TGF beta1 (Transforming Growth Factor Beta) - Single Plex is used for the simultaneous quantification of the following Mouse, Human, Rat, Pig, Horse, Rabbit, Guinea Pig, Hamster analytes: TGF-β1
The MILLIPLEX® MAP Phospho Akt1/PKBα (Ser473) MAPmate is used for the simultaneous quantification of the following Human analytes: Akt (PKB), Akt/PKB (Ser473) - AB1
The MILLIPLEX® MAP Phospho Erk/MAP Kinase 1/2 (Thr185/Tyr187) MAPmate is used for the simultaneous quantification of the following Human analytes: Erk/MAPK 1/2, Erk/MAPK 1/2 (Thr185/Tyr187)
The MILLIPLEX® MAP Phospho EGF Receptor MAPmate is used for the simultaneous quantification of the following Human analytes: EGFR, EGFR (pan Tyr)
The MILLIPLEX® MAP Active Caspase 3 MAPmate is used for the simultaneous quantification of the following Human analytes: Caspase 3, Caspase 3 (Active)
The MILLIPLEX® MAP Total Akt/PKB MAPmate is used for the simultaneous quantification of the following Human analytes: Akt (PKB), Akt/PKB (Total) - AB1
The MILLIPLEX® MAP Total EGF Receptor MAPmate is used for the simultaneous quantification of the following Human analytes: EGFR, EGFR (Total)
The MILLIPLEX® MAP Phospho HSP27 (Ser78) MAPmate is used for the simultaneous quantification of the following Human analytes: HSP27, HSP27 (Ser78)
The MILLIPLEX® MAP Total HSP27 MAPmate is used for the simultaneous quantification of the following Human analytes: HSP27, HSP27 (Total)
The MILLIPLEX® MAP Total Erk/MAP Kinase 1/2 MAPmate is used for the simultaneous quantification of the following Human analytes: Erk/MAPK 1/2, Erk/MAPK 1/2 (Total)
