Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
The ES Cell Marker Sample Kit includes six monoclonal antibodies for the analysis of ES cell marker expression. CHEMICON®'s ES Cell Marker Sample Kit (Catalog number SCR002) contains monoclonal antibodies for the detection of the cell-surface Stage-Specific Embryonic Antigens (SSEA-1, SSEA-3 & SSEA-4), expression of TRA-1-60, TRA-1-81 antigens and Oct-4. This kit permits the phenotypic analysis of the differentiation status of…
EMD Millipore's Alkaline Phosphatase Detection Kit is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
EMD Millipore's Mesenchymal Stem Cell Characterization Kit (Catalog Number SCR018) contains a panel of positive and negative selection markers for the characterization of the mesenchymal stem cell population in rat. Positive cell markers include antibodies directed against cell-surface molecules (integrin beta1 and CD54) (Pittenger, M. F., and Marshak, D. R., & Pittenger, M.F., et al.) present on mesenchymal stem cells along w…
CHEMICON®'s Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining. Reagents in the kit include two ECM coating molecules (collagen Type I and vitronectin) that have been shown to promote osteogenic differentiation of mesenchymal stem cells (Salasznyk, 2004) along with inducing reagents, dexamethaso…
The Rodent Neuron Differentiation Kit is a system designed for the optimal differentiation of neural stem cells derived from rodents into neurons. Included in the kit are two neural inducers that when added to the Neural Stem Cell Basal Medium allows for the preferential differentiation of rodent neural stem cells to a neuronal lineage. Other components of the kit include reagents for the fixation and two antibodies for the im…
The Pancreatic Islet Cell Characterization Kit (SCR045) provides a convenient set of validated antibodies that allows researchers to reliably identify mature pancreatic islets cells. Along with antibodies generated against discrete hormones secreted by alpha, beta, delta and gamma cells of the pancreatic islets, the kit includes PDX-1 (pancreatic duodenal homeobox gene-1), a master regulator of islet cell development and GLUT-…
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by…
TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.
