Human CRISPRi Kinase Phosphatase Drug Targets Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in.
The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.
Pooled CRISPR screening with 10x genomics compatibility for discovery at single cell resolution
Apoptosis, or programmed cell death, is a highly regulated pathway that is important in normal developmental processes as well as many diseases. Cells undergoing apoptosis are identifiable by a number of characteristics, including changes in mitochonrial membrane potential, transport of phosphatidylserine (PS) to the membrane surface, activation of caspase proteases, and DNA fragmentation in the nucleus. Guava offers four apop…
Guava Technologies' ViaCount assay is revolutionizing the way cell counting and viability assessment are done in today's laboratories. Fast, automated, and highly reproducible, the Guava ViaCount assay addresses many of the difficulties of conventional cell counting and viability assessment methods. The Guava ViaCount assay provides: Cell counts up to ten times faster than manual counting, in a convenient turnkey assay.…
The new Guava Nexin® reagent monitors induction of apoptosis quickly and easily, with minimal chance for contamination. Annexin V is a calcium-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), a membrane component normally localized to the internal face of the cell membrane. Early in the apoptotic pathway, molecules of PS are translocated to the outer surface of the cell membrane where Ann…
Millipore's full-length mTOR kinase is now available as part of the KinaseProfiler™ Service. This kinase is the first biologically relevant mTOR to be commercially available. The KinaseProfiler Service offers 264 kinases for selectivity profiling, using a catalytic radiometric assay format. The addition of the full-length mTOR to the KinaseProfiler panel provides high-quality, confidential screening of compounds against this c…
Millipore now proudly offers the MILLIPLEX® MAP Human Cancer Biomarker Panel. The Cancer Biomarker Panel includes both a Five-Plex assay, which simultaneously measures five serum biomarkers, Macrophage Migration Inhibitory Factor (MIF), Prolactin, CA-125, Leptin and Osteopontin (OPN), and a Single Plex IGF-II assay. The combination of the six serum biomarkers that discriminate between disease-free and ovarian cancer patien…
Guava Mitochondrial Depolarization Assay for detection of early apoptosis. There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility. Guava Technologies has optimized an…
The Guava Caspase Assay Suite of fluorescent-based assays for Caspase 3/7, 8, 9 and multi-caspase employ the same protocol making it easier to perform multiple caspase assays in parallel. Further, Guava caspase reagents are membrane permeable allowing one to measure intracellular levels of the enzyme without using harsh lysis methods. The Guava Caspase Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspase) reagent th…
New Release 2009! 3D Prime Culture Medium. Improving still further our originial medium for 3D culture of epidermal keratinocytes, CELLnTEC has now released a new formulation, known as 3D Prime.Developed using our established signalling-based approach, this completely defined medium enables more rapid establishment of a fully stratified epidermis. Using this medium, models can now be ready for experimentation in just 14-18 day…
CnT-27 is a progenitor cell targeted (PCT) liquid culture medium kit including both basal medium and separate supplements, developed for improved isolation and growth of mammary epithelium. CnT-27 is fully defined, serum and BPE-free formulation. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency. Suitable fo…
High Content Analysis (HCA) is rapid, automated microscopy providing quantitative image acquisition and analysis. Using Millipore’s superior-quality reagents in ready-to-use kits and cell-based assays that incorporate the power of HCA, researchers around the world are able to learn more about relevant functional cell biology in many different systems, including assessment of DNA damage and apoptosis. Millipore’s HCA kits inclu…
Components of the Upstate (Millipore) Casein Kinase 2 Assay Kit:• Casein Kinase 2 Substrate Peptide (Cat.# 12-330) • Assay Dilution Buffer I (ADBI) (Cat.# 20-108) • PKA Inhibitor Cocktail (Cat.# 20-114) • Magnesium/ATP Cocktail (Cat.# 20-113) • P81 Phosphocellulose Squares (Cat.# 20-134) For use to measure the phosphotransferase activity of Casein Kinase 2 (CK-2) in immunoprecipitates and column fractions based on phosphorylat…
