Drug discovery > Target Discovery Products & Reviews

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The quality of sequence obtained from Next Generation sequencing platforms such as Illumina, Inc. requires the accurate determination of input library DNA. Loading too much DNA can lead to overlapping clusters and loss of read counts whereas too little input can lead to poor read lengths and lower sequence output. The PureGenome™ NGS Library Validator Kit measures the absolute amount of adapter-ligated, sequence ready DNA in I…

A sterile, closed, disposable system for sampling fluids from sterile and aseptic processes The unique NovaSeptum sampling system is ideal for general fluid and cell culture sampling from aseptic and sterile processes. Both systems can be configured for high purity or autoclavable sampling applications and as single or multiple sampling units. A wide range of holders and connectors are also available. •  Closed sampling elimin…

GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents…

BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extracti…

AVOID FREEZE/THAW CYCLES. The ProteoExtract Subcellular Proteome Extraction Kit is a tool for the differential extraction of proteins from adherent or suspension tissue culture cells according to their subcellular localization. The sequential extraction steps yield fractions containing cytosolic proteins, plasma membrane and organelle proteins, soluble nuclear proteins, and finally cytoskeletal and nuclear matrix proteins. To…

PhosphoSafe™ Extraction Buffer extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. The reagent, an enhancement of the CytoBuster™ Protein Extraction reagent, includes four phosphatase inhibitors. PhosphoSafe is compatible with Western blotting, immunoprecipitations, and kinase assays.  

Pellet Paint® NF Co-Precipitant* is a non-fluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has o…

Overnight Express™ Instant TB Medium is a complete granulated culture medium for high-level protein production without the need to monitor cell growth in the pET and other IPTG-inducible bacterial expression systems. Cell mass and target protein yield are often increased several-fold as compared with induction using IPTG. The method is based on media components that are metabolized differentially to promote growth to high dens…

White solid. Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3¡ä-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Intro…