PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:
PMEF-HL are Hygromycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded before freezing for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
PMEF-NL are Neomycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use. See ES Stem Cell Manual for complete culture and media for PMEF cells.
RESGRO Culture Medium is a complete ready-to-use product that can be utilized to complement traditional murine Embryonic Stem (ES) cell culture media containing ESGRO® murine LIF medium supplement. In contrast to routine ES cell culture using ESGRO® supplemented medium, RESGRO Culture Medium is recommended for a number of specialized applications.
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. When used in conjunction with bFGF or bFGF, EGF and heparin, the basal medium will allow for the proliferation of rat and mouse neural stem cells, respectively. Withdrawal of the growth factors from the Neural Stem Cell Basal Medium w…
The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
MilliTrace Mouse Embryonic Stem Cell Expansion Medium is a complete ready-to-use expansion medium optimized for the culture of MilliTrace GFP Reporter Mouse Embryonic Stem Cells. Puromycin Solution is provided to help maintain the expression of the green fluorescent protein (GFP) labeled transgene.
The Cardiac Stem Cell Maintenance Medium has been optimized and qualified for the growth and expansion of cardiac stem cells derived from rodent origins. Cells expanded in Cardiac Stem Cell Maintenance Medium express the correct cardiac stem cell markers and are furthermore capable of differentiating into cardiomyocytes.
The PluriStem™ 129S6 Murine ES cell line is derived from the widely used 129/S6/SvEv strain of mice (at 3.5 days p.c.) and is intended for injection into black or albino host blastocysts. This cell line is characterized by the production of highly chimeric, germline-competent mice. Cells are supplied at passage 10.
CHEMICON®'s Cryopreserved Adult Rat Hippocampal Neural Stem Cells provides ready-to use primary neural stem cells isolated from the hippocampus of adult Fisher 344 rats. These primary cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders. For Research Use Only; not…
EMD Millipore's Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.…
ESGRO Complete™ Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF and BMP, and during routine washing steps when passaging cells.
ESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.
Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 12. This ES cell line has been pre-adapted to serum-free and feeder-free cell culture conditions and is intended for use with the ESGRO…
A highly efficient method of gene trasfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene with DMSO shock is also used to mediate DNA transfer into a variety of cell types, such as: CHO, chicken embryo fibroblases, NIH3T3 cells, and myeloid cells.
DNA Extraction Kit is a simple, non-toxic and efficient way to isolate high molecular weight genomic DNA from a wide variety of mammalian cell sources including: whole blood, body fluids, bone marrow, isolated mononuclear cells, tissue culture cells and solid tissues. Sufficient reagents are supplied for 20 DNA extractions of 2.5-5.0 x 107 cells or 40 DNA extractions of 2.5 x 107 cells. The kit can also be used for apoptotic D…
CHEMICON's EX-WAX™ DNA Extraction Kit is intended to extract DNA from paraffin-embedded tissue fixed in 10% Formalin or non-crosslinking fixatives. DNA extracted by this kit is suitable for amplification by PCR.
Upstate's new Nitrotyrosine Assay Kit, Chemiluminescence Detection is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including white high binding 96-well plates, nitrated BSA standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers. The assay has a wide dynamic range and high precision, making…
Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Ra…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The AKT plate is coated with a specific mouse monoclonal anti-AKT capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Akt plate is coated with a specific mouse monoclonal Akt capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incub…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal anti-EGFR capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal EGFR capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR antig…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Src plate is coated with a specific mouse monoclonal anti-Src capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…
