This cell line arose from a primary culture of dispersed human fetal pancreatic cells. A single pancreas gland at 18 weeks gestation was dispersed by micro-dissection and collagenase digestion (2 mg/ml for 50 minutes at 37°C). Following washout of enzyme, dispersed cells were incubated with magnetic beads coupled to monoclonal antibodies specific to CD90 though a DNA linkage. Magnetic isolation and the detachment of the magnet…
Embryonic Stem Cell Lines derived from murine 129/SVEV selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 11.
Embryonic Stem Cell Lines derived from murine strain C57/BL6J selected for high targeting efficiency and proven germ line transmission. Cells are supplied as 2.5 x 106 ES cells per vial (approximately 2.5 population doublings per passage, shipped frozen on dry ice) and are supplied at passage 11.
This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data)and is ES cell qualified. Freezing Media contain 10% of the active cryopreservation ingredient (either DMSO or Glycerol). The remaining 90% is a proprietary mixture of Calf and Fetal Bovine Serum. The reagents used in these products are certified to be virus and mycoplasma free. The sera ut…
The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS). Such sera must be carefully controlled and tested. Specialty Media utilizes sera that are approved by the United States Department of Agriculture (USDA) to ensure that sera are free of diseases such as Bovine Spongiform Encephalopathy (BSE). A Certificate…
The maintanence, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS). Such sera must be carefully controlled and tested. EMD Millipore utilizes sera that are approved by the United States Department of Agriculture (USDA) to ensure that sera are free of diseases such as Bovine Spongiform Encephalopathy (BSE). A Certificate of…
We now offer our most popular embryo culture media in a dry powder format. Each has been formulated for optimal, consistent performance. This new dry powdered format offers greatly increased shelf-life, easy and quick preparation, and the freshest nutrients to support the development of your embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with…
Dry Powdered form offers easy and quick preparation, and the freshest nutrients to support the development of embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with the correct pH and osmolarity.
dry powder format. Each has been formulated for optimal, consistent performance. This new dry powdered format offers greatly increased shelf-life, easy and quick preparation, and the freshest nutrients to support the development of your embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with the correct pH and osmolarity.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
PMEF-CFL are Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:
PMEF-HL are Hygromycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded before freezing for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
PMEF-NL are Neomycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use. See ES Stem Cell Manual for complete culture and media for PMEF cells.
RESGRO Culture Medium is a complete ready-to-use product that can be utilized to complement traditional murine Embryonic Stem (ES) cell culture media containing ESGRO® murine LIF medium supplement. In contrast to routine ES cell culture using ESGRO® supplemented medium, RESGRO Culture Medium is recommended for a number of specialized applications.
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. When used in conjunction with bFGF or bFGF, EGF and heparin, the basal medium will allow for the proliferation of rat and mouse neural stem cells, respectively. Withdrawal of the growth factors from the Neural Stem Cell Basal Medium w…
The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
MilliTrace Mouse Embryonic Stem Cell Expansion Medium is a complete ready-to-use expansion medium optimized for the culture of MilliTrace GFP Reporter Mouse Embryonic Stem Cells. Puromycin Solution is provided to help maintain the expression of the green fluorescent protein (GFP) labeled transgene.
The Cardiac Stem Cell Maintenance Medium has been optimized and qualified for the growth and expansion of cardiac stem cells derived from rodent origins. Cells expanded in Cardiac Stem Cell Maintenance Medium express the correct cardiac stem cell markers and are furthermore capable of differentiating into cardiomyocytes.
The PluriStem™ 129S6 Murine ES cell line is derived from the widely used 129/S6/SvEv strain of mice (at 3.5 days p.c.) and is intended for injection into black or albino host blastocysts. This cell line is characterized by the production of highly chimeric, germline-competent mice. Cells are supplied at passage 10.
CHEMICON®'s Cryopreserved Adult Rat Hippocampal Neural Stem Cells provides ready-to use primary neural stem cells isolated from the hippocampus of adult Fisher 344 rats. These primary cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders. For Research Use Only; not…
EMD Millipore's Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.…
ESGRO Complete™ Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF and BMP, and during routine washing steps when passaging cells.
ESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.
