Merck Products & Reviews

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Recombinant Cas9-D10A Nickase protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments.

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Human CRISPRi Kinase Phosphatase Drug Targets Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in.

The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Pooled CRISPR screening with 10x genomics compatibility for discovery at single cell resolution

Certified reference material, Trace CERT ® , 100 μg/mL each component in methanol, ampule of 1 mL.

Platform can perform automatic analysis of sample's purity, identification and degradation of compounds through calibrated algorithm-based digital references.

For researchers who want to do cell cycle research at their benchtop, Guava Cell Cycle assay can be used for measuring G0/G1, S, and G2/M phase distributions. Based on the established method of whole-cell staining with propidium iodide (PI), Guava's cell cycle assay is easy to learn. Guava Cell Cycle software includes markers that can be set for quick assessment of G0/G1, S, and G2/M phase cell cycle percentages as data is bei…

Apoptosis, or programmed cell death, is a highly regulated pathway that is important in normal developmental processes as well as many diseases. Cells undergoing apoptosis are identifiable by a number of characteristics, including changes in mitochonrial membrane potential, transport of phosphatidylserine (PS) to the membrane surface, activation of caspase proteases, and DNA fragmentation in the nucleus. Guava offers four apop…

Guava Technologies' ViaCount assay is revolutionizing the way cell counting and viability assessment are done in today's laboratories. Fast, automated, and highly reproducible, the Guava ViaCount assay addresses many of the difficulties of conventional cell counting and viability assessment methods. The Guava ViaCount assay provides: Cell counts up to ten times faster than manual counting, in a convenient turnkey assay.…

The new Guava Nexin® reagent monitors induction of apoptosis quickly and easily, with minimal chance for contamination. Annexin V is a calcium-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), a membrane component normally localized to the internal face of the cell membrane. Early in the apoptotic pathway, molecules of PS are translocated to the outer surface of the cell membrane where Ann…

Millipore's full-length mTOR kinase is now available as part of the KinaseProfiler™ Service. This kinase is the first biologically relevant mTOR to be commercially available. The KinaseProfiler Service offers 264 kinases for selectivity profiling, using a catalytic radiometric assay format. The addition of the full-length mTOR to the KinaseProfiler panel provides high-quality, confidential screening of compounds against this c…

Millipore's new ArthroMAB™ Monoclonal Antibody Cocktail is widely used to generate arthritis mouse models. These models are used for rapid screening and evaluation of anti-inflammatory therapeutics. ArthroMAB™ initiates arthritis via immune complex formation on the surface of cartilage in joints. ArthroMAB™ is a cocktail of four monoclonal antibodies directed against Collagen. It rapidly induces robust arthritis in all four…

Millipore now proudly offers the MILLIPLEX® MAP Human Cancer Biomarker Panel. The Cancer Biomarker Panel includes both a Five-Plex assay, which simultaneously measures five serum biomarkers, Macrophage Migration Inhibitory Factor (MIF), Prolactin, CA-125, Leptin and Osteopontin (OPN), and a Single Plex IGF-II assay. The combination of the six serum biomarkers that discriminate between disease-free and ovarian cancer patien…

The Fast-Trap Lentivirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification o…

The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification o…

Mouse embryonic stem cells (mESCs) are unique cells in that they can be maintained in vitro as pluripotent cells and can be induced to differentiate into many cell types by manipulating culture conditions. mESCs have been used as a model to help define differentiation and signaling pathways in human embryonic stem cells (hESCs), since hESCs are more arduous to work on. Therefore, much attention has been focused on understandin…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell Characterization kit is designed as an easier and quicker way to track surface marker expression of hOCT4, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both hOCT4 and SSEA4 and not SSEA1. This quick test will allow researchers to determine th…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell Surface Marker Characterization kit is designed to facilitate an easier and quicker way to track surface marker expression of TRA-1-60, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both TRA-1-60 and SSEA4 and not SSEA1. This quick test will a…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell New Surface Marker Characterization Kit is designed to facilitate an easier and quicker way to track surface marker expression of HESCA1, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both HESCA1 and SSEA4 and not SSEA1. This quick test will a…

Millipore’s The FlowCellect™Rodent Neural Stem Cell Characterization Kit for Astrocyte Differentiation is designed as an easier and quicker way to track Astrocyte differentiation. The kit will also help to determine the percentage of undifferentiated rat neural stem cells in culture by cells that express both Sox-2 and Nestin as well as track GFAP upregulation upon Astrocyte differentiation. This quick test will allow research…

Millipore’s FlowCellect™ GPCR Surface Identification kit is designed to provide an easier and more efficient way of characterizing GPCR expression on a given cell type of interest. This kit includes optimized buffers and a high affinity, specific antibody capable for detecting the GPCR target in question. Also included in the kit is a secondary antibody conjugate to R-Phycoerythrin, which is a bright fluorochrome ideal for mea…

Millipore’s FlowCellect™ GPCR Surface Identification kit is designed to provide an easier and more efficient way of characterizing GPCR expression on a given cell type of interest. This kit includes optimized buffers and a high affinity, specific antibody capable for detecting the GPCR target in question. Additional test applications include the identification of high, medium, and low expressing cell cultures during the clonal…