dry powder format. Each has been formulated for optimal, consistent performance. This new dry powdered format offers greatly increased shelf-life, easy and quick preparation, and the freshest nutrients to support the development of your embryos in culture. Just add the sterile diluent (included in the kit) to the powder, mix gently, and filter to prepare FRESH medium with the correct pH and osmolarity.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
PMEF-CFL are Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:
PMEF-HL are Hygromycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded before freezing for later use.
PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels
PMEF-NL are Neomycin resistant Primary Mouse Embryo Fibroblasts that have NOT been mitotically arrested. The cells are provided at passage 3 and can be further expanded for about 5-6 passages and frozen for later use. See ES Stem Cell Manual for complete culture and media for PMEF cells.
RESGRO Culture Medium is a complete ready-to-use product that can be utilized to complement traditional murine Embryonic Stem (ES) cell culture media containing ESGRO® murine LIF medium supplement. In contrast to routine ES cell culture using ESGRO® supplemented medium, RESGRO Culture Medium is recommended for a number of specialized applications.
The Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. When used in conjunction with bFGF or bFGF, EGF and heparin, the basal medium will allow for the proliferation of rat and mouse neural stem cells, respectively. Withdrawal of the growth factors from the Neural Stem Cell Basal Medium w…
The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
