Chemicon’s cloned human CCR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR1 expression on the cell surface and contains high levels of the promiscuous G protein G15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR1 and its ligands.
Chemicon's CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.
Chemicon's cloned human CCR3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR3 and its ligands.
Chemicon's cloned human CCR4-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR4 expression on the cell surface and contains high levels of the promiscuous G protein Ga15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR4 and its ligands.
EMD Millipore's cloned rhesus macaque CCR5-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR5 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR5 and its ligands.
EMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium respon…
Chemicon's cloned human CCR7-expressing cell line is made in the Chem-2 host, which supports high levels of recombinant CCR7 expression on the cell surface and contains high levels of the promiscuous G protein Galpha16 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR7 and its ligands.
Chemicon's cloned human CX3CR1 -expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CX3CR1 expression on the cell surface and contains high levels of promiscuous G proteins to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CX3CR1 and its ligands.
Chemicon's CX3CR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of fractalkine/CX3CR1 interactions. The membrane preparations exhibit a Kd of 0.05 nM for [125I]-fractalkine. With 2.5 mg/well CX3CR1 Membrane Prep and 0.05 nM [125I]-fractalkine, a greater tha…
Chemicon's cloned human C5a receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant C5aR expression on the cell surface and contains high levels of the promiscuous G protein Ga15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of C5a/C5aR interactions.
