Clinical diagnostics encompasses the detection, diagnosis, and monitoring of diseases and health conditions in patients. Utilizing advanced lab techniques and medical technologies, clinical scientists and healthcare professionals analyze samples such as blood, urine, and tissue to identify biomarkers, pathogens, and other indicators of illness. From point of care testing (POCT) to precision medicine approaches, clinical diagnostics plays a crucial role in early intervention, treatment planning, and disease management. Explore how this field empowers clinicians to deliver accurate diagnoses and improve patient outcomes.
As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradient solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
To interface the µPAC™ column with the Easy-spray source, a customized EASY-Spray™ Emitter was developed, which is now distributed by PharmaFluidics. To ensure straightforward and plug and spray connectivity, this emitter line has been provided with a low volume stainless steel connecting union that fits directly onto the µPAC™ column fittings.
Our µPAC™ Trapping column was developed with an identical stationary phase support morphology, matching perfectly the retentive properties of our analytical µPAC™ columns. Effectively desalting and preconcentrating the analytes improves the analytical column lifetime and workflow throughput.
Because the column is meanly made out of silicon which is semi-conductive, a dramatic impact on the chromatographic behavior of our µPAC™ columns has been observed if no appropriate precautions are taken in case the column is connected to an ESI source of a mass spectrometer. Therefore we developed a custom made grounding cable to shunt the high voltage to ground.
The µPAC™ capLC columns are designed for increased robustness and throughput assuring sensitivity. A flow rate versatility between 1 and 15 µL/min at moderated pressures allows short gradient separations, while the µPAC™ technology ensures an exceptional high reproducibility over time and across laboratories. µPAC™ column, 50cm length, with pillar array backbone at interpillar distance of 2.5 µm, C18.
