As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradient solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
To interface the µPAC™ column with the Easy-spray source, a customized EASY-Spray™ Emitter was developed, which is now distributed by PharmaFluidics. To ensure straightforward and plug and spray connectivity, this emitter line has been provided with a low volume stainless steel connecting union that fits directly onto the µPAC™ column fittings.
Our µPAC™ Trapping column was developed with an identical stationary phase support morphology, matching perfectly the retentive properties of our analytical µPAC™ columns. Effectively desalting and preconcentrating the analytes improves the analytical column lifetime and workflow throughput.
Because the column is meanly made out of silicon which is semi-conductive, a dramatic impact on the chromatographic behavior of our µPAC™ columns has been observed if no appropriate precautions are taken in case the column is connected to an ESI source of a mass spectrometer. Therefore we developed a custom made grounding cable to shunt the high voltage to ground.
The µPAC™ capLC columns are designed for increased robustness and throughput assuring sensitivity. A flow rate versatility between 1 and 15 µL/min at moderated pressures allows short gradient separations, while the µPAC™ technology ensures an exceptional high reproducibility over time and across laboratories. µPAC™ column, 50cm length, with pillar array backbone at interpillar distance of 2.5 µm, C18.
