Separations is a fundamental scientific discipline focused on the isolation and purification of components from mixtures. Through techniques such as chromatography, distillation, and filtration, separations scientists separate substances based on their physical or chemical properties. This field plays a crucial role in various industries, including pharmaceuticals, food and beverage testing, environmental science, materials research, and more. Explore how separations techniques enable precise analysis, quality control, and the development of innovative products.
As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
For scientists running complex LCMS assays in low flow chromatography, the 50 cm µPAC™ GEN2 nanoLC column provides exceptional reproducible results over time and across laboratories through its µPAC™ Column technology and further enhances chromatographic separation with its second generation pillars design.
With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradient solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
To interface the µPAC™ column with the Easy-spray source, a customized EASY-Spray™ Emitter was developed, which is now distributed by PharmaFluidics. To ensure straightforward and plug and spray connectivity, this emitter line has been provided with a low volume stainless steel connecting union that fits directly onto the µPAC™ column fittings.
Our µPAC™ Trapping column was developed with an identical stationary phase support morphology, matching perfectly the retentive properties of our analytical µPAC™ columns. Effectively desalting and preconcentrating the analytes improves the analytical column lifetime and workflow throughput.
Because the column is meanly made out of silicon which is semi-conductive, a dramatic impact on the chromatographic behavior of our µPAC™ columns has been observed if no appropriate precautions are taken in case the column is connected to an ESI source of a mass spectrometer. Therefore we developed a custom made grounding cable to shunt the high voltage to ground.
Our µPAC™ columns are provided with 1/32” fittings to be compatible with any third party LC system. To connect the column into a 1/16” valve an additional capillary containing 1/16” pre-assembled fittings is provided.
The µPAC™ capLC columns are designed for increased robustness and throughput assuring sensitivity. A flow rate versatility between 1 and 15 µL/min at moderated pressures allows short gradient separations, while the µPAC™ technology ensures an exceptional high reproducibility over time and across laboratories. µPAC™ column, 50cm length, with pillar array backbone at interpillar distance of 2.5 µm, C18.
The µPAC™ Flex iON Connect is a user-friendly interface to facilitate fast, reproducible and low dispersion connection of µPAC ™ nanoLC columns to Thermo Fisher Scientific Nanospray Flex™ Series ion sources. It is compatible with any type of 360µm OD electrospray emitters through one-piece fingertight fitting and it embeds a preassembled liquid junction configuration to achieve the best performance.
The µPAC™ GEN2 Limited Sample Amount column is designed to maximize protein identifications when working with limited sample amounts.
