Visitron VisiTIRF Wide Field Fluorescence Imaging
Photon Lines LtdThe TIRF technique is a special kind of wide-field fluorescence technique for measuring cellsor SMC in an evanescent field without haze. By total reflection of the exciting light (typicallylaser) at the coverslip / medium interface, the fluorescence excitation is limited to a very thin space in the vicinity of the glass surface. The resulting fluorescence images exhibit extremely high contrast and resolution with the possibili…