Products & Reviews

Supelco provides high quality derivatization reagents for akylation and esterification.Alkylation is the replacement of the active hydrogen in R-COOH, R-OH, R-SH, R-NH, R-NH2, R-CONH2, R-CONH-R′ with an alkyl group, or sometimes an aryl group.Esterification is the first choice for derivatization of carboxylic acids and other acidic functional groups. Acids are reactive compounds, and are too polar to be well separated by gas c…

Supelco® has developed a line of high performance fittings that maintain the integrity of your HPLC systems up to 15,000 psi with mere finger tightening!The use of HPLC systems to achieve faster analyses and increased resolution pushes systems toward higher operating pressures. Our selection of Supel™-Connect High Performance Interconnects and Fittings will assist the chromatographer in maximizing system performance and reliab…

Supelco’s complete line of stock and custom packed GC columns are configured to fit most commercially available GC instruments.Supelco® has been the leader in packed GC column technology for over 40 years. Should you not be able to locate a stock item to fit your needs, our custom capabilities allow us to manufacture columns and packings to your specifications. In addition we also carry hard-to-find tools specifically designed…

High Capacity Gas Purifiers (Remove Moisture and Oxygen)The best purifier choice for removing moisture and oxygen from carrier gas streams is our High Capacity Gas Purifier. It consists of three parts:• Clam-shell oven• Replaceable heating elements• Replaceable converter tube.A convenient starter kit contains all three items for new installations.

Supelcarb® is Back by Popular Demand!Supelco® is pleased to welcome the Supelcarb HC Hydrocarbon trap back to our line of in-line gas purifiers! Supelcarb adsorbent has the greatest trapping ability of hydrocarbons per gram of adsorbent (twice that of activated charcoal). These traps are the best traps available for removing hydrocarbons and other organics from carrier, fuel, air and other gas streams.

Specialty In-Line Purifier for GC-MS HeliumThe most sensitive GC-MS instruments require a steady stream of ultra-high purity (99.99999%) helium for optimal performance. To achieve this level, a specialty purifier is required. These purifiers contain multiple adsorbent beds for the removal of all critical contaminants. Additionally, the adsorbent materials are packed into the cartridge under helium to ensure no other gas off-g…

Purification Consumables and Custom Products.We understand that your purification challenges are unique. That’s why we offer you a variety of options to help you solve them. Whether you seek the up-front cost savings and versatility of packing your own columns, the consistent quality and time savings of pre-packed columns, or the ease associated with having us purify material for you; Sigma-Aldrich is your purification partner…

HPLC and FPLC Columns for the Analysis and Purification of Biopolymers.Biopolymers, including peptides, proteins, polynucleotides, and other biodegradable polymers, display a variety of ionic charges and differ in terms of hydrophobicity, size, shape and mass. In addition to these structural (and functional) complexities, an individual biopolymer is typically present at low concentration in samples that contain many similar co…

Cyclodextrin-based DEX and Astec CHIRALDEX phases are stable, high boiling liquids and make effective CSPs for enantiomer separations by GC. The two lines exhibit complementary selectivity.Cyclodextrins are macromolecules composed of 6 or more D(+)-glucose residues bonded through α-glycosidic linkages. They are classified according to the number of glucose residues they contain: α-cyclodextrins (six residues), β-cyclodextrins…

Supelco′s new Low Adsorption (LA) vials are manufactured using a process that decreases the number of hydroxyl groups on the vial′s glass surface, significantly reducing surface activity while improving analytical quantitation and minimizing pH shifts in the sample.This same process also removes unwanted surface metals such as sodium and boron that can contaminate samples and interfere with trace analysis. Unlike other methods…

Our new certified vial, cap and septa products eliminate "fit" and septa contamination concerns associated with other brands.By taking these extra manufacturing and testing steps, we can ensure:• Caps thread properly onto vials, providing an air-tight seal• Syringe needles are not damaged by irregular vial bottom thickness• Minimal contamination from septa impurities.Quality is built into each component of the Supelco certifie…

The pentafluorophenylpropyl (PFP) stationary phase of Ascentis® Express F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, some…

This column allows highly reproducible analyses of fatty acid methyl esters (FAMEs), specifically the omega 3 and omega 6 fatty acids. It is tested to ensure reproducible FAME equivalent chain length (ECL) values and resolution of key components. Column Details: Phase: Bonded; poly(ethylene glycol) USP Code: G16 Length: 15, 30 m ID: 0.10, 0.25, 0.32, 0.53 mm df: 0.10, 0.25, 0.50

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users. Column Details: Mode of chromatography: Hydrophilic interaction…

Application: The 35% phenyl equivalent phase provides a higher polarity option compared to columns containing a lower phenyl content, resulting in a greater retention of polar analytes relative to non-polar compounds. Its selectivity is complementary when paired with SLB-5ms for applications that require dual-column confirmatory analysis, such as for environmental analytes (such as pesticides, PCBs, and herbicides). The low…

The Next Generation in Hybridspe-Phospholipid Technology

The Ascentis Express biphenyl phase chemistry offers alternative selectivity for pharmaceutical analytes and drug metabolites that are not well retained or difficult to resolve on traditional alkyl (C18) and phenyl bonded phases. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 ?m particles while maintaining lower backp…

The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…

Patent pending HybridSPE – Precipitation (HybridSPE-PPT) technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then…