Separations Products & Reviews

Separations is a fundamental scientific discipline focused on the isolation and purification of components from mixtures. Through techniques such as chromatography, distillation, and filtration, separations scientists separate substances based on their physical or chemical properties. This field plays a crucial role in various industries, including pharmaceuticals, food and beverage testing, environmental science, materials research, and more. Explore how separations techniques enable precise analysis, quality control, and the development of innovative products.

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HPLC and FPLC Columns for the Analysis and Purification of Biopolymers

Sigma-Aldrich Supelco

HPLC and FPLC Columns for the Analysis and Purification of Biopolymers.Biopolymers, including peptides, proteins, polynucleotides, and other biodegradable polymers, display a variety of ionic charges and differ in terms of hydrophobicity, size, shape and mass. In addition to these structural (and functional) complexities, an individual biopolymer is typically present at low concentration in samples that contain many similar co…

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Chiral Capillary GC Columns

Sigma-Aldrich Supelco

Cyclodextrin-based DEX and Astec CHIRALDEX phases are stable, high boiling liquids and make effective CSPs for enantiomer separations by GC. The two lines exhibit complementary selectivity.Cyclodextrins are macromolecules composed of 6 or more D(+)-glucose residues bonded through α-glycosidic linkages. They are classified according to the number of glucose residues they contain: α-cyclodextrins (six residues), β-cyclodextrins…

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Low Adsorption (LA) - Neutral pH Vials

Sigma-Aldrich Supelco

Supelco′s new Low Adsorption (LA) vials are manufactured using a process that decreases the number of hydroxyl groups on the vial′s glass surface, significantly reducing surface activity while improving analytical quantitation and minimizing pH shifts in the sample.This same process also removes unwanted surface metals such as sodium and boron that can contaminate samples and interfere with trace analysis. Unlike other methods…

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Certified Vials

Sigma-Aldrich Supelco

Our new certified vial, cap and septa products eliminate "fit" and septa contamination concerns associated with other brands.By taking these extra manufacturing and testing steps, we can ensure:• Caps thread properly onto vials, providing an air-tight seal• Syringe needles are not damaged by irregular vial bottom thickness• Minimal contamination from septa impurities.Quality is built into each component of the Supelco certifie…

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Ascentis® Express F5 HPLC Columns

Sigma-Aldrich Supelco

The pentafluorophenylpropyl (PFP) stationary phase of Ascentis® Express F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, some…

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Omegawax® Capillary GC Columns

Sigma-Aldrich Supelco

This column allows highly reproducible analyses of fatty acid methyl esters (FAMEs), specifically the omega 3 and omega 6 fatty acids. It is tested to ensure reproducible FAME equivalent chain length (ECL) values and resolution of key components. Column Details: Phase: Bonded; poly(ethylene glycol) USP Code: G16 Length: 15, 30 m ID: 0.10, 0.25, 0.32, 0.53 mm df: 0.10, 0.25, 0.50

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Supelclean™ EZ-POP NP

Sigma-Aldrich Supelco

This multi-bed solid phase extraction (SPE) cartridge was designed for the extraction of non-polar persistent organic pollutants (POPs), specifically heavy and light polynuclear aromatic hydrocarbons (PAHs), from edible oil matrices such as olive and corn oil. Fatty matrix is preferentially retained by the cartridge while PAHs are washed through using acetonitrile. The resulting extract is suitable for either GC-MS or HPLC-FL…

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Supel™ QuE Z-Sep QuEChERS Tubes

Sigma-Aldrich Supelco

Supel QuE Z-Sep sorbents enhance sample cleanup for complex matrices by effectively removing more fat and color from sample extracts than traditional phases for QuEChERS. By eliminating problematic matrix interferences, Z-Sep products provide more robust LC-MS and GC-MS methods. This proprietary technology can replace C18 and PSA phases in your current methods without additional method development.Supel QuE Z-Sep/C18, a combi…

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SupelMIP® SPE - Patulin

Sigma-Aldrich Supelco

The SupelMIP SPE line consist s of highly cross-linked polymers that are engineered to extract a single analyte of interest or a class of structurally related analytes with an extremely high degree of selectivity. This is possible because selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chem…

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Ascentis® Express HILIC 2.7 Micron HPLC Column

Sigma-Aldrich Supelco

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users. Column Details: Mode of chromatography: Hydrophilic interaction…

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