Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
More than 18,000 studies have been published citing the use of the yeast two-hybrid system to investigate protein-protein interactions, and Clontech’s Matchmaker™ Systems have been by far the most popular. Matchmaker™ Gold Systems are the highest performing incarnations of our Matchmaker product line, due to a combination of a new yeast strain (Y2HGold), stringent reporters, easy to use libraries, high-level expression vectors…
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…
This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for interca…
Research and Clinical Grade RetroNectin Reagent for Retroviral or Lentiviral Transduction. RetroNectin Reagent for retroviral or lentiviral transduction significantly increases transduction efficiency and results in enhanced gene transfer, especially in suspension and hematopoietic stem cells. RetroNectin Reagent is a chimeric peptide composed of E. coli-derived recombinant human fibronectin fragment that contains three funct…
Flow Cytometer Calibration Beads for AcGFP/EGFP and mCherry. Use these beads to calibrate your flow cytometer prior to analyzing cells that express the AcGFP1 or mCherry fluorescent proteins. The AcGFP Flow Cytometer Calibration Beads allow for easy calibration of any flow cytometer with a 488 nm laser line that excites the green fluorescent proteins AcGFP1 (Aequorea coerulescens GFP) and EGFP. [The AcGFP Flow Cytometer Calib…
DEXPAT Reagent from Takara Bio is designed for one step DNA extraction from paraffin embedded tissue fixed in 10% formalin. DEXPAT Reagent eliminates the laborious deparaffinization, organic solvent extraction and enzymatic digestion steps required in most standard DNA extraction protocols. Furthermore, DEXPAT Reagent enables preparation of PCR-ready DNA in only 25 minutes versus 2-3 days using conventional DNA extraction met…
The EpiScope MSP Kit features a reagent designed exclusively for DNA methylation analysis via methylation specific PCR (MSP). This reagent contains a specific enzyme in an optimized buffer, allowing MSP analyses on bisulfite-treated uracil-containing DNA templates. This kit features high amplification efficiency and methylation specificity compared with standard PCR. The EpiScope MSP Kit is optimized for real-time monitoring…
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantag…
The SMARTer Stranded RNA-Seq Kit provides a solution for generating libraries for Illumina® sequencing platforms that retain strand information with >99% accuracy.
SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian—Strand-Specific RNA-Seq Libraries for Illumina Platforms from High-Input Total RNA. The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina® sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines key RiboGone and SMART technologies, seamlessly blending efficient rRNA removal and str…
