Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
Thermo Scientific Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5´ phosphates from DNA and RNA and is completely and irreversibly inactivated by heating at 65°C for 15 minutes. SAP is active on 5´ overhangs, 5´ recessed and blunt ends.
Thermo Scientific X-Gal (5-bromo-4-chloro-3-indoyl-ß-D-galactopyranoside) is a chromogenic substrate used to identify recombinant plasmids. In the presence of X-Gal and IPTG, lac+ bacterial colonies appear blue, whereas recombinant colonies appear white.
The Thermo Scientific 96-Well Semi-Skirted Plates, Fast Block hold a maximum of 0.1 mL of well volume when used in conjunction with adhesive and heat seals. The plates are directly compatible with all Applied Biosystems thermal cycler fast blocks, no adapters necessary. These semi-skirted low profile thin walled plates feature a raised deck to aid in spill prevention and come with a cut corner at the A1 location. The 96-Well S…
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Random Decamers are truly random oligo deoxyribonucleotides that can be used to create a pool of cDNAs for subsequent applications, thus providing maximum flexibility.
Thermo Scientific Random Hexamers are truly random oligo deoxyribonucleotides. Hexamers are ideal primers for mRNA with or without a poly-A tail. Hexamers bind more often than decamers, giving higher yields and increased sensitivity when amplifying short fragments.
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Magnesium ions (Mg2+) are an important component of PCR reactions where they interact with the DNA template, dNTPs, and Taq DNA Polymerase.
Adhesive film and foils seals for PCR and qPCR plates. Gas permeable seals and crystallography seals. Adhesive PCR Film Seal:Thermo Scientific Adhesive PCR Plate Seals are a reliable method of sealing plates for PCR applications. The high integrity sealing materials give the best protection against evaporation for high efficiency PCR. Highlights • Clear for sample inspection • Strong adhesive prevents evaporation during therm…
Power SYBR® Green RNA-to-CT 2-Step Kit is a flexible kit that enables a two-step protocol for performing reverse transcription and cDNA quantitation by real-time PCR. The kit includes the High Capacity RNA-to-cDNA and the PowerSYBR® Green PCR Master Mix. The High Capacity RNA-to-cDNA Kit is used to first reverse transcribe RNA into cDNA and the resulting cDNA is then quantitated using the PowerSYBR® Green PCR Master Mix for…
Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.
Ambion Pre-miR™ miRNA Precursor Molecules are small, chemically-modified double-stranded RNA molecules designed to mimic endogenous mature miRNA molecules. These ready-to-use miRNA mimics, which are similar to but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters identical to those used for siRNAs and enable detailed study of miRNA biological effects via gain-of-function e…
