Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over.
The Human Regulatory T cell Staining Kit #1 contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies sufficient for 25 tests: eZFluor™ Anti-Human CD4 FITC and CD25 PE Cocktail (clones RPA-T4 and BC96), Anti-Human Foxp3 APC (clone PCH101) Rat IgG2a…
The Human Regulatory T cell Staining Kit #2 contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies sufficient for 25 tests: eZFluor™ Anti-Human CD4 FITC and CD25 APC Cocktail (clones RPA-T4 and BC96, Anti-Human Foxp3 PE (clone PCH101), Rat IgG2a…
The Human Regulatory T cell Staining Kit #3 contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies sufficient for 25 tests: eZFluor™ Anti-Human CD4 FITC and CD25 PE Cocktail (clones RPA-T4 and BC96), Anti-Human Foxp3 PE-Cyanine5 (clone PCH101), R…
The Human Regulatory T Cell Whole Blood Staining Kit contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3 cells in whole blood samples. The Anti-Human Foxp3 PE (PCH101) monoclonal antibody reacts with the amino terminus of human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors.…
The Mouse Regulatory T cell Staining Kit #1 contains buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify mouse regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies: Anti-Mouse CD4 FITC (RM4-5), Anti-Mouse CD25 APC (PC61.5), Anti-Mouse/Rat Foxp3 PE (FJK-16s), Rat IgG2a K Isotype Control PE, Anti-Mouse CD16/32 Purifi…
The Mouse Regulatory T cell Staining Kit #1 contains buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify mouse regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies: Anti-Mouse CD4 FITC (RM4-5), Anti-Mouse CD25 PE (PC61.5), Anti-Mouse/Rat Foxp3 APC (FJK-16s), Rat IgG2a K Isotype Control APC, Anti-Mouse CD16/32 Purif…
The Mouse Regulatory T cell Staining Kit #1 contains buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify mouse regulatory T cells from human peripheral blood cells. The kit contains the following labeled monoclonal antibodies: Anti-Mouse CD4 FITC (RM4-5), Anti-Mouse CD25 PE (PC61.5), Anti-Mouse/Rat Foxp3 PE-Cy5 (FJK-16s), Rat IgG2a K Isotype Control PE-Cy5, Anti-Mouse CD16/32…
JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes coloras membrane potentials increase (over values of about 80-100 mV). When excited at 488 nm, the emission shift goes from 530 nm (monomeric JC-1) to 590 nm (aggregate JC-1), which are easily detected using filter…
eFluor 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. When it enters a cell, cellular esterases cleave the dye, resulting in a membrane-impermeable dye that fluoresces at ~520 nm. eFluor™ 514 Calcium Sensor Dyeis a commonly used dy…
