Drug discovery > Basic Research Products & Reviews

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

Method completed in less than one day versus 2 days with restriction enzymes

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.

Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.

Restriction Enzymes Dpn I is an ideal enzyme for cloning and Southern blot analysis High purity enzyme preparation Includes 10X universal reaction buffer Glogos II Autorad Markers Luminescent peel-off stickers used for accurately marking and aligning autoradiographs. Easily affixed to plastic wrap or paper, these convenient markers emit low-level luminescence rather than radioactivity, making the Glogos II m…

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.

Our SideStep lysis and Stabilization buffer ensures accurate gene expression studies by eliminating sample loss and degradation. Our proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Following the 10-minute room temperature lysis, you can go directly from cells to QPCR amplification without stopping to purify. 

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.

Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible features. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.  

The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.

Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element  

Determine the direct or indirect involvement of new gene products, growth factors, and drug candidates in specific pathways with our PathDetect Trans-Reporting Systems.  Easily investigate whether your protein or compound influences the activation of any of the six transcription factors offered.  These systems are available as plasmid vectors with a luciferase reporter for co-transfection with your gene of interest into the c…

The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.

Hygromycin B is an antibiotic that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…

The MBS Mammalian Transfection kit can increase your transfection efficiencies up to 100-fold. Cells are incubated with CaPO 4 /DNA coprecipitate for only 3 hours, versus 6 to 12 hours with traditional CaPO 4 methods. Additionally, cells can be assayed 12 to 24 hours post-transfection instead of the usual 48 to 72 hours.  

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind ® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution.