Drug discovery > Basic Research Products & Reviews

Ampicillin, IPTG, and X-Gal in convenient packaging.  G418 Sulfate  Hybromycin B

Agilent X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, BCIG) is a premium quality chromogenic substrate of β-Galactosidase. It is used with cloning systems that utilize β-galactosidase activity as an indicator of successful cloning.

The Agilent miRNA Spike-in kit allows researchers to monitor the miRNA labeling and hybridization reactions. The Spike-in kit contains a labeling and hybridization spike-in, which correspond to complementary probe sequences on the microarrays. The Spike-ins and probes are designed using Drosophila.

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.  New cDNA synt…

The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay.  More sensitive than traditional assays (use as few as 10e4 cells)  Enhancer is non-cytotoxic and non-denaturing  Up to 40-fold signal enhancement for cultured cells  Proprietary enhancer which increases signal at lea…

The Mitochondrial membrane potential detection kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential. In healthy non-apoptotic cells, the dye stains the mitochondria bright red.  Detect the loss in mitochondrial membrane potential - Early indicator of cellular apoptosis  Convenient kit format  Use with flow cytometer, fluorescence plate reader, fluorescence microscope

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…

The Agilent Oligo aCGH Prehybridization Buffer is used to wet and condition arrays for hybridization on a Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. It provides for better distribution of the sample over the active area, and helps prevent the occurrence of gradients in the signals.

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).  Detects protein-prote…

The InterPlay TAP Purification Buffer Kit is designed to work with the InterPlay Mammalian TAP System and InterPlay Adenoviral TAP System to recover interacting proteins from mammalian cells. The InterPlay TAP Purification Buffer Kit includes the buffers required to perform tandem affinity purification.

Agilent's GeneSpring provides powerful, accessible statistical tools for intuitive data analysis and visualization. 

SureCall is a research desktop application combining both novel and widely accepted opensource algorithms for end-to-end NGS data analysis from alignment to categorization and annotation of mutations, supporting Agilent Target Enrichment applications. 

The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. 

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

A collection of classic strains and competent cells.

The ABLE C strain and the ABLE K strain help solve issues associated with toxic clones. These strains reduce the copy number of common cloning vectors by ~4- and 10-fold, enhancing the probability that a toxic clone will be propagated.

The ABLE C strain and the ABLE K strain help solve issues associated with toxic clones. These strains reduce the copy number of common cloning vectors by ~4- and 10-fold, enhancing the probability that a toxic clone will be propagated.

The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells.  In situ staining of cells directly in dishes without removing or manipulating cells  Rapid detection of Ÿ-gal activity in transfected cells  Results in 24 hours  Maximum signal with low background  Deep blue staining reaction  Kit in…

Perform high sensitivity protein electrophoresis with the 2100 Bioanalyzer System. The High Sensitivity Protein 250 assay provides a broad dynamic range with sensitivity down to Silver Stain comparable levels. Efficiency is enhanced by eliminating handling of SDS-PAGE slab gels, staining or, imaging steps.

Perform high sensitivity protein electrophoresis with the 2100 Bioanalyzer System. The High Sensitivity Protein 250 assay provides a broad dynamic range with sensitivity down to Silver Stain comparable levels. Efficiency is enhanced by eliminating handling of SDS-PAGE slab gels, staining or, imaging steps.

The Supercoiled DNA Ladder contains fragments from 2 kb to 10 kb at 1-kb increments and an additional band at 12 kb. Each supercoiled band is at a known concentration, with the 7-kb band at a higher concentration than the others. The resulting brighter band in the middle of the ladder acts as a useful reference point.  Accurate size determination facilitated by evenly spaced bands  Fragments are exact multiples of 1000 bp…