Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
The CHEMICON® In Vitro Vascular Permeability Assay is performed in a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain 1.0 μm symmetrical pores within a transparent polyethylene membrane. The high pore density membranes permit amplified rates of basolateral diffusion with molecules of interest for permeability assays. The membrane is tissue culture treated on both sides for cell growth. Within CHE…
The CHEMICON® Endothelial Cell Adhesion Assay kit (ECM645) allows the researcher to test a variety of cell types that interact with activated or inactivated endothelial cell layers. The CHEMICON® Endothelial Cell Adhesion Assay kit includes a 96 well tissue culture treated fluorescence microtiter plate and reagents that allow for large-scale screening and quantitative comparison of multiple samples/cell line adhesion to an en…
ECM675 uses purified chicken type I collagen in 6mM acetate buffer pH 7.0. The product is 99.9% pure native atelomeric avian collagen. Approximately 85% type I and 15% type III collagen protein. The material has been specially prepared to maintain its native state as much as possible. The telomeric ends of the collagen I have been enzymatically removed. Since most of the species identity is found within the telomeric ends, our…
The Multidrug Resistance Direct Dye Efflux Assay Kit includes two of the best characterized and most commonly used multidrug resistance ABC transporter substrates, DiOC2(3) and rhodamine 123. The kit enables researchers to directly assess the functional activity of the MDR1, MRP1 and BCRP membrane pumps in living cells under physiologic conditions by directly measuring the relative fluorescence of cell populations that activel…
CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.
Chemicon's Mast Cell Degranulation Assay Kit provides a simple and convenient means for assaying tryptase activity. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate tosyl-gly-pro-lys-pNA. The free pNA can then be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. A pNA Standard is provided as a comparison control.
CHEMICON's NS220 Neurite Outgrowth Assay Kit (3 µm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm diameter, e.g. N1E-115 cells [Abe et al., 2003], Dorsal Root Ganglia and…
CHEMICON's NS225 Neurite Outgrowth Assay Kit (1 μm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsibl…
ApopTag® Equilibration Buffer is intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
