Drug discovery > Basic Research Products & Reviews

Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.

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Sure Blot® Nylon Membrane - Roll

Merck

Sure Blot® Hybridization Membrane is a positively charged nylon membrane for rapid ionic bonding of nucleic acids. Due to its high nucleic acid retention capacity, Sure Blot® can be stripped and reprobed up to 10 times without altering its performance characteristics. A proprietary casting process ensures uniform surface characteristics and lot-to-lot consistency.

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Sure Blot® CHEMI™ Hybridization & Detection Kit

Merck

CHEMICON's Sure Blot® CHEMI™ Hybridization & Detection Kit is designed to provide high quality, non-radioactive detection of digoxigenin-labeled DNA probes hybridized to Sure Blot® Nylon Membrane. As an integrated hybridization and detection system, Sure Blot® CHEMI™ is quality controlled to produce high signal and low background. Comparable sensitivity to 32P is typically observed for single-copy gene detection. Sufficient re…

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Rho Assay Reagent (Rhotekin RBD, agarose)

Merck

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.

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Rho Activation Assay Kit

Merck

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity 90%.

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Tyrosine Kinase Assay Kit, Colorimetric Detection

Merck

This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the wel…

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