Identification and optimization of the chemical, series of chemicals or bio-molecule that will interact with the target using impartial, expert opinion about the best equipment, services and the latest techniques
Chemicon's EP3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of EP3 interactions and its ligands. The membrane preparations exhibit a Kd of 1.56 nM for [3H]-PGE2. With 1.5 nM [3H]-PGE2, 5 µg/well and 10 µg/well EP3 Membrane Prep typically yield greater than…
Chemicon's cloned human GAL1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GAL1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between GAL1 and its ligands.
Chemicon's cloned human kappa-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant kappa expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of the kappa receptor.
Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of κ interactions with diprenorphine. The membrane preparations exhibit a Kd of 2.3-3.7 nM for [3H]-diprenorphine. With 10 µg/well κ Membrane Prep and 2.5 nM [3H]-diprenorphine, a greater than 3-fold…
Chemicon's cloned human--2A-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant alpha2A expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between-alpha2A and its ligands.
Chemicon's cloned human S1P3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.
EMD Millipore's S1P3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P3 interactions with its ligands. The membrane preparation exhibits EC50 of 1.7nM for S1P in a GTPγS binding assay.
EMD Millipore’s cloned human NMU2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NMU2 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at NMU2.
Chemicon's cloned human EP1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant EP1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between EP1 and its ligands.
Chemicon's cloned human δ opioid - expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of the δ opioid receptor
Chemicon's δ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at δ. The membrane preparations exhibit a Kd of 1.12 nM for [3H]-DADLE. With 0.9 nM [3H]-DADLE, 10 µg/well δ Membrane Prep typically yields greater than 4-fold signal-to-background rati…
Chemicon's µ opioid receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of µ opioid receptor interactions with its ligands. The membrane preparations exhibit an EC50 of 1.6nM for DAMGO in a GTPγS binding assay.
Chemicon's cloned human D1-expressing cell line is made in the Chem-6 host, which supports high levels of recombinant D1 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between D1 and its ligands.
Chemicon's D3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D3 interactions and its ligands. The membrane preparations exhibit a Kd of 0.1 nM for [125I]-Iodospiperone. With 5.0ug/well D3 membrane prep and 0.1 nM [125I]-Iodospiperone, a greater than 4-fol…
EMD Millipore's β1 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β1 adrenoceptor interactions. The membrane preparations exhibit a Kd of 2.6 nM for [125I]-(-) Iodocyanopindolol (ICYP). With 5 µg/well β1 Adrenoceptor Membrane Prep and 0.25 nM…
Chemicon's cloned human 5-HT1A-expressing cell line is made in the Chem-3 host, which supports high levels of recombinant 5-HT1A expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between 5-HT1A and its ligands.
EMD Millipore's 5-HT1B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT1B. The membrane preparations exhibit a Kd of 10.5 nM for [3H]-GR125743. With 7 nM [3H]-GR125743, 10μg/well 5-HT1B Membrane Prep typically yields greater than 8-fold si…
Chemicon's cloned human 5-HT2B -expressing cell line is made in the CHO host, which supports high levels of recombinant 5-HT2B expression on the cell surface. Thus, the cell line is an ideal tool for screening for antagonists of interactions between 5-HT2B and its ligands.
Chemicon's cloned human Glucagon Receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Glucagon Receptor expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between the Glucagon Receptor and its ligands…
Chemicon's glucagon receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of glucagon receptor interactions and its ligands. The membrane preparations exhibit a Kd of 3.09 nM for [125I]-glucagon. With 1 nM [125I]-glucagon, 5 or 10 μg/well glucagon receptor…
Chemicon's cloned human M2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant M2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between M2 and its ligands.
Chemicon's cloned human M4 -expressing cell line is made in the Chem-4 host, which supports high levels of recombinant M4 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between M4 and its ligands.
Chemicon's cloned human GABAB1b/GABAB2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GABAB1b/GABAB2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between GABAB and its ligands.
Chemicon's GABAB1b membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GABAB1b receptor interactions with its ligand. The membrane preparations exhibit a Kd of 1.15nM for [3H]-CGP 54626A. With 5.0 ug/well GABAB1b Membrane Prep and 7.5nM [3H]-CGP 54626A, a grea…
Chemicon's cloned human SST4-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant SST4 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between the SST4 and its ligands.
