Identification and optimization of the chemical, series of chemicals or bio-molecule that will interact with the target using impartial, expert opinion about the best equipment, services and the latest techniques
Chemicon's cloned human GPR54-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR54 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of GPR54.
Chemicon's GPR14 Membrane Preparations are ideal tools for screening for antagonists of urotensin II/GPR14 interactions. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-urotensin II. With 10 mg/well GPR14 Membrane Prep and 0.3 nM [125I]-urotensin II, a greater than 20-fold signal-to-background ratio is obtained.
Chemicon's cloned human NTR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NTR1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between NTR1 and neurotensin.
Chemicon's cloned human CCR9-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR9 expression on the cell surface and contains high levels of the promiscuous Galpha15 protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR9 and its ligands.
Chemicon's CCR9 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR9 interactions with its ligands. The membrane preparations exhibit an EC50 of 8 nM for TECK/CCL25 in a GTPgammaS binding assay.
Chemicon's PAR2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PAR2 interactions with its ligands. The membrane preparations exhibit an EC50 of 1.9 μM for furoyl-LIGRLO in a GTPγS binding assay.
Chemicon's cloned human D2L-expressing cell line is made in the Chem-7 host, which supports high levels of recombinant D2 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between D2 and its ligands.
EMD Millipore’s cloned human D2L-expressing cell line is made in the CHO host, which supports optimal levels of recombinant D2L expression for robust agonist-induced cAMP signal. Thus, the cell line is an ideal tool for screening for agonists and antagonists at the D2L Receptor.
EMD Millipore's D2 dopamine membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D2 dopamine receptor interactions with its ligand. The membrane preparations exhibit a Kd of 0.51nM for [125I]-Iodospiperone. With 5.0 ug/well D2 Dopamine Membrane Prep and 0.1nM […
Chemicon's VPAC1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC1 interactions with VIP. The membrane preparations exhibit a Kd of 1.2 nM for [125I]-VIP. With 2.5 μg/well VPAC1 Membrane Prep and 0.4 nM [125I]-VIP, a greater than 5-fold signal-to-backg…
