Identification and optimization of the chemical, series of chemicals or bio-molecule that will interact with the target using impartial, expert opinion about the best equipment, services and the latest techniques
Chemicon's β2 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β2 adrenoceptor interactions with (-)Iodocyanopindolol (ICYP). The membrane preparations exhibit a Kd of 0.51 nM for [125I]-(-)ICYP. With 5 µg/well β2 Adrenoceptor Membrane Prep and…
Chemicon's cloned human PK1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PK1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PK1 and its ligands.
Chemicon's cloned human S1P2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P2 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P2 and its ligands
Chemicon's S1P2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P2 interactions with its ligands. The cell line exhibits EC50 of 7.2nM for S1P in a GTPγS binding assay.
Chemicon's VPAC2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC2 interactions with PACAP. The membrane preparations exhibit a Kd of 0.9 nM for [125I]-PACAP27. With 5 µg/well VPAC2 Membrane Prep and 0.5 nM [125I]-PACAP27, a greater than 15-fold signal…
Chemicon's cloned human 5-HT2A -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant 5-HT2A expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between 5-HT2A and its ligands.
Chemicon's α1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of α1A interactions with prazosin. The membrane preparations exhibit a Kd of 1.34 nM for [3H]-prazosin. With 5 µg/well α1A Membrane Prep and 1 nM [3H]-prazosin, a greater than 7-fold signal-to-bac…
Chemicon's cloned human LPA1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between LPA1 and its ligands.
EMD Millipore's LPA1 Membrane Preparation is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for EMD Millipore’s LPA1 Calcium Optimized Cell Line (HTS089C). This LPA1 Membrane Preparation exhibits both constitutive and agonist-induced activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist, inverse agonist, and antagonist activi…
EMD Millipore’s cloned human EP3 -expressing cell line is made in the Chem-1 host which stably expresses a mitochondrially targeted flash mutant form of aequorin. The purified version of this flash variant of aequorin has shown a higher luminescent signal intensity than purfied wildtype aequorin. Thus, the cell line is an ideal tool for screening for antagonists of interactions between EP3 and its ligands.
