Identification and optimization of the chemical, series of chemicals or bio-molecule that will interact with the target using impartial, expert opinion about the best equipment, services and the latest techniques
miVac is a range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. miVac concentrators come in 2 sizes, the smaller Duo and a larger Quattro. There is a choice of vacuum pump and rotor according to application and sample format. To speed concentration built-in special runs for working with water and…
Double-beam, variable wavelength capability Programmable wavelengths: 190 - 900 nm East-to-use Control Pad Available Lamp startup/shutdown Pre-aligned lamps for easy changeover Remote autozero capability Optimized for use with ESA Coulometric electrochemical detection Low noise and low drift for increasing sensitivity and stability in analyses. The Model 526/528 detectors provide extremely low operating noise levels…
A low volume electrochemical cell optimized for the low flow rates of electrospray ionization mass spectrometry (ESI-MS). • Optimized for incorporation into low flow rate LC-MS applications • Provides high efficiency electrochemical conversion with minimal peak dispersion Proven Coulometric DesignThe Model 5030 Cell utilizes the proven porous graphite flow through design pioneered by ESA and is optimized for incorporation…
An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…
A rapid and sensitive homogeneous assay for cortisol assessmentThe HTRF® cortisol assay can be used for the quantification of cortisol in rodent serum, or directly on cells. This assay has also been validated for 11-beta HSD1 screening on whole cells or microsomes.The assay requires only 2 reagent additions, followed by an incubation period of 2 hours at room temperature. It has a detection limit of 70 pg/mL (e.g. 2 fmol/well…
A straightforward tool for biomolecular labeling with Europium cryptateThe Cryptate labeling kit includes all the necessary materials to allow rapid Cryptate labeling of various biomolecules and purification. The labeling reagent Eu3+-TBP-NHS Cryptate is suitable for labeling peptides, proteins, and oligonucleotides, as well as small molecules containing amino groups (N-terminus or lysine residues). The labeling procedure requ…
A range of HTRF® conjugates for assay developmentHTRF® toolbox assembles a panel of detecting reagents such as Streptavidin-XLlent!, anti-tag antibodies like Eu-Cryptate anti-GST, 6HIS, DNP and anti species conjugated antibodies offering many possibilities for the study of molecular mechanisms. All reagents are available as Cryptate-Europium or XL665 (the two HTRF tracers) conjugates, to allow easy assay development. These re…
Streamlined single plate protocols including all stimulation and cytokine quantification.The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynamics, and no use of isotopes. Procedures are performed on a single plate with simultaneous cell stimulation and cytokine detection. They can all be run under a similar experimental procedure stream…
A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stab…
A fast and reliable process for biomolecule labelingIn many circumstances, assay development and HTS involve specific labeling of the various assay components. Cisbio international’s labeling service is an excellent alternative for the labeling of proteins, peptides, oligonucleotides, or other biomolecules either with Cryptate, XL665, biotin or DNP when you do not have the time or the facilities to label them yourself.Two type…
