Products, services, reviews and techniques used in lead molecule delivery, formulation and toxicology, using assays and animal models.
Cell Line Description: The ToxReporter XRE Cell Line contains a reporter gene under transcriptional control of the Xenobiotic Response Element (XRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the XRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can th…
Cell Line Description: The ToxReporter HRE Cell Line contains a reporter gene under transcriptional control of the Hypoxia Response Element (HRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the HRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can then…
Cell Line Description: The ToxReporter Hmox1 Cell Line contains a reporter gene under transcriptional control of the Hemoxygenase Response Element (Hmox1) promoter. This reporter construct is stably integrated into the CHO cell line. The induction of a toxic response through the Hmox1 pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media…
ToxReporter Cell Line Starter Pack 9 is required for use with the Hmox1 ToxReporter Cell Line (Catalog # ECL-009). In response to toxic compounds, ToxReporter proteins are secreted from cells into culture media. This secreted protein may be measured using the ToxReporter ELISA kit (Catalog # ETR-201K).
The ToxReporter Cell Line Starter Pack 2 (Catalog # ECLSP-002) includes ToxReporter AP-1 Stimulant (Catalog # ESTM-002), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately.
The ToxReporter Cell Line Starter Pack 5 (Catalog # ECLSP-005) includes ToxReporter NFĸB Stimulant (Catalog # ESTM-005), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately.
The ToxReporter Cell Line Starter Pack 3 (Catalog # ECLSP-003) includes ToxReporter GRE Stimulant (Catalog # ESTM-003), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter™ GRE Cell Line Starter Pack is required for use with the GRE ToxReporter Cell Line (Catalog #…
The ToxReporter Cell Line Starter Pack 1 (Catalog # ECLSP-001) includes ToxReporter p21 Stimulant (Catalog # ESTM-001), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter™ p21 Cell Line Starter Pack is required for use with the p21 ToxReporter Cell Line (Catalog #…
The ToxReporter Cell Line Starter Pack 4 (Catalog # ECLSP-004) includes ToxReporter HSP70 Stimulant (Catalog # ESTM-004), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter™ HSP70 Cell Line Starter Pack is required for use with the HSP70 ToxReporter Cell Line (Cat…
The ToxReporter Cell Line Starter Pack 8 (Catalog # ECLSP-008) includes ToxReporter ARE Stimulant (Catalog # ESTM-008), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter Cell Line Starter Pack 8 is required for use with the ARE ToxReporter Cell Line (Catalog # EC…
One vial of ToxReporter XRE Stimulant (Catalog # ESTM-007) contains 100 µL of 100 µM 3-methylchoranthrene in 10% DMSO at 100X concentration. One vial of ToxReporter Selection Agent (Catalog # ESA-001) contains 20 mL of proprietary selection compound at 100X concentration. One vial of Assay Media Supplement (Catalog # EAMS-001) contains 1 mL of proprietary reagent at 100X concentration. ToxReporter Cell Line Starter Pack 7 is…
The ToxReporter Cell Line Starter Pack 6 (Catalog # ECLSP-006) includes ToxReporter HRE Stimulant (Catalog # ESTM-006), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter Cell Line Starter Pack 6 is required for use with the HRE ToxReporter Cell Line (Catalog # E…
This ToxReporter Cell Line is used in conjunction with the ToxReporter ELISA kit (cat# ETR-201K) for the measurement of ToxReporter protein secreted into cell culture media as a consequence of exposure to a potential toxin. The cell line has been designed to respond to stimuli that induce cellular stress response pathways. Examples of stressful stimuli include compounds in drug discovery and organic or inorganic molecules pote…
This ToxReporter Cell Line is used in conjunction with the ToxReporter ELISA kit (cat# ETR-201K) for the measurement of ToxReporter protein secreted into cell culture media as a consequence of exposure to a potential toxin. The cell line has been designed to respond to stimuli that induce cellular stress response pathways. Examples of stressful stimuli include compounds in drug discovery and organic or inorganic molecules pote…
This ToxReporter Cell Line is used in conjunction with the ToxReporter ELISA kit (cat# ETR-201K) for the measurement of ToxReporter protein secreted into cell culture media as a consequence of exposure to a potential toxin. The cell line has been designed to respond to stimuli that induce cellular stress response pathways. Examples of stressful stimuli include compounds in drug discovery and organic or inorganic molecules pote…
This ToxReporter Cell Line is used in conjunction with the ToxReporter ELISA kit (cat# ETR-201K) for the measurement of ToxReporter protein secreted into cell culture media as a consequence of exposure to a potential toxin. The cell line has been designed to respond to stimuli that induce cellular stress response pathways. Examples of stressful stimuli include compounds in drug discovery and organic or inorganic molecules pote…
This ToxReporter Cell Line is used in conjunction with the ToxReporter ELISA kit (cat# ETR-201K) for the measurement of ToxReporter protein secreted into cell culture media as a consequence of exposure to a potential toxin. The cell line has been designed to respond to stimuli that induce cellular stress response pathways. Examples of stressful stimuli include compounds in drug discovery and organic or inorganic molecules pote…
This kit is used for the non-radioactive quantification of ToxReporter protein that is secreted into cell culture media. One kit is sufficient to measure 38 unknown samples in duplicate.
LifeXtend™ conjugate stabilizer/diluent is a proprietary multi-component reagent system that stabilizes antibody-HRP conjugates both at 4°C and at ambient temperature. By using LifeXtend™ HRP stabilizer/diluent in antibody conjugation the HRP conjugate can be stabilized during use and stored as pre-diluted, ready-to-use reagents thus eliminating waste and improving consistency from experiment to experiment. LifeXtend™ shows…
Experience Better Productivity and Save Resources Too RapidFire data correlates identically with traditional LC-MS/MS methods Analyses are performed at a 10+ fold increase in cycle throughput, enabling us to provide 3 day turnaround RapidFire In Vitro ADME Analysis Services increase efficiencies while preserving your lab’s assay method integrity. We use the RapidFire 360 high-throughput TOF system to analyze CYP inhibition/ind…
This kit enables simultaneous measurements in the same cell of six independent parameters that monitor cell health, including cell loss, nuclear size and morphological changes, mitochondrial membrane potential changes, cytochrome c release, and changes in cell permeability.
The BrdU and Ki67 Cell Proliferation HCS Reagent Kits are for simultaneous quantification of DNA replication and Ki67 proliferation marker in the same cell.
The Multiparameter Cell Death Detection Kits for high-content screening (HCS) enable simultaneous quantitation of LC3B protein on autophagic vesicles, cytochrome c localization and its release from mitochrondria, cell membrane integrity and its permeability and nuclear morphology and DNA content.
The micronucleus assay is an important component of genetic toxicology screening programs. Micronuclei arise from acentric chromosomes and lagging whole chromosomes.
The Autophagy & Proteasome Function: LC3B and Poly-Ubiquitin Detection Kits enable quantitation of LC3B protein on autophagic vesicles and ubiquitin on polyubiquitinated proteins.
