Drug discovery > Pre-Clinical Development Products & Reviews

Millipore’s FlowCellect™ GPCR Surface Identification kit is designed to provide an easier and more efficient way of characterizing GPCR expression on a given cell type of interest. This kit includes optimized buffers and a high affinity, specific antibody capable for detecting the GPCR target in question. Also included in the kit is a secondary antibody conjugate to R-Phycoerythrin, which is a bright fluorochrome ideal for mea…

Millipore’s FlowCellect™ GPCR Surface Identification kit is designed to provide an easier and more efficient way of characterizing GPCR expression on a given cell type of interest. This kit includes optimized buffers and a high affinity, specific antibody capable for detecting the GPCR target in question. Also included in the kit is a secondary antibody conjugate to R-Phycoerythrin, which is a bright fluorochrome ideal for mea…

Guava Mitochondrial Depolarization Assay for detection of early apoptosis. There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility. Guava Technologies has optimized an…

For researchers who want to do cell cycle research at their benchtop, the Guava® Cell Cycle Assay can be used for measuring G0/G1, S, and G2/M phase distributions. Based on the established method of whole-cell staining with propidium iodide (PI), the Guava Cell Cycle Assay is easy to learn. Guava Cell Cycle Software includes markers that can be set for quick assessment of G0/G1, S, and G2/M phase cell cycle percentages as data…

The Guava Caspase Assay Suite of fluorescent-based assays for Caspase 3/7, 8, 9 and multi-caspase employ the same protocol making it easier to perform multiple caspase assays in parallel. Further, Guava caspase reagents are membrane permeable allowing one to measure intracellular levels of the enzyme without using harsh lysis methods. The Guava Caspase Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspase) reagent th…

Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate - Rat IL-1/IL-1F2 Immunoassay for the quantitative determination of rat interleukin 1 beta (IL-1ß) concentrations in cell culture supernates, rat serum, and rat EDTA plasma. The R&D Quantikine Rat IL-1 beta ELISA is a 4.5 hour solid phase ELISA. This R&D ELISA employs the quantitative sandwich enzyme immunoassay technique. R&D Quantikine ELISA Kits are complete and ready-to-…

Human Total MMP-9 Quantikine ELISA Kit- Human MMP-9 (total) Immunoassay for the quantitative determination of human active and pro-Matrix Metalloproteinase 9 (total MMP-9) concentrations in cell culture supernates, saliva, serum, plasma, and urine.The R&D Quantikine MMP-9 Immunoassay is a 3.5 hour solid phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique. R&D Systems Quantikin…

R&D Human sVCAM-1/CD106 Quantikine ELISA Kit - Human sVCAM-1 Immunoassay for the quantitative determination of human soluble Vascular Cell Adhesion Molecule-1 (sVCAM-1) concentrations in cell culture supernates, serum and plasma. The Quantikine Human sVCAM-1 ELISA is a 2 hour solid-phase ELISA. This R&D Quantikine assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sVCAM-1h…

R&D Human IGF-I Quantikine ELISA Kit - Human IGF-I Immunoassay for the quantitative determination of human insulin-like growth factor I (IGF-I) concentrations in cell culture supernates, serum, and plasma. R&D Systems Quantikine ELISA Kits are complete and ready-to-use. Immunoassays are available for measuring a range of molecules including cytokines, chemokines, growth factors, proteases, and more. The R&D Quantikine Human I…

R&D Systems Mouse/Rat/Porcine TGF-beta 1 Quantikine ELISA Kit - Mouse/Rat/Porcine/Canine TGF-beta Immunoassay for the quantitative determination of activated mouse/rat/porcine/canine Transforming Growth Factor beta 1 (TGF-ß 1) concentrations in cell culture supernates, serum, and plasma.The Quantikine TGF-beta ELISA is a 4.5 hour solid phase ELISA. This R&D quantikine assay employs the quantitative sandwich enzyme immunoassay…

R&D Human IFN-gamma Quantikine ELISA Kit - Human IFN-gamma Immunoassay for the quantitative determination of human interferon gamma (IFN-gamma) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. The Quantikine ELISA contains E. coli-expressed recombinant…

R&D Mouse TNF-alpha/TNFSF1A DuoSet This R&D DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Tumor Necrosis Factor alpha (TNF-alpha). DuoSets are designed for the analysis of cell culture supernates. Other sample types, such as serum and plasma, need to be validated prior to use in this R&D DuoSet. Each R&D DuoSet ELISA kit cont…

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…

VEGF (Vascular endothelial growth factor) is a potent polypeptide regulator of blood vessel function. VEGF promotes an array of responses in the endothelium, including hyperpermeability, endothelial cell growth, angiogenesis, and enhanced glucose transport. Several alternate splice isoforms of VEGF are known. Two high-affinity receptors for VEGF, VEGFR1 and VEGFR2, have been identified and characterized. Key features of this…

Fits plastic or glass media bottles with 45mm neck sizes. Cellulose acetate (CA) membranes provide fast flow rates and low protein binding and are good for filtering cell culture media. Angled hose connector simplifies vacuum line attachment. Individually packaged, sterile and certified nonpyrogenic. Each system has the membrane material and pore size printed on the unit and is color-coded by membrane type for easy produ…

R&D Fluorokine Receptor Detection Kits - Biotinylated ligands for estimating receptor densities R&D Systems now offers a Human Activin A Biotinylated Fluorokine Kit (Catalog # NFACTA). This flow cytometry kit is designed for the detection of Activin A receptor-expressing cells by flow cytometry. Activin A is a disulfide-linked homodimer belonging to the TGF-beta superfamily that signals through Type I/Type II receptor comple…

Cell Line Description: The ToxReporter ARE Cell Line contains a reporter gene under transcriptional control of the Antioxidant Response Element (ARE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the ARE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can t…

Cell Line Description: The ToxReporter XRE Cell Line contains a reporter gene under transcriptional control of the Xenobiotic Response Element (XRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the XRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can th…

Cell Line Description: The ToxReporter HRE Cell Line contains a reporter gene under transcriptional control of the Hypoxia Response Element (HRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the HRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can then…

Cell Line Description: The ToxReporter Hmox1 Cell Line contains a reporter gene under transcriptional control of the Hemoxygenase Response Element (Hmox1) promoter. This reporter construct is stably integrated into the CHO cell line. The induction of a toxic response through the Hmox1 pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media…

ToxReporter Cell Line Starter Pack 9 is required for use with the Hmox1 ToxReporter Cell Line (Catalog # ECL-009). In response to toxic compounds, ToxReporter proteins are secreted from cells into culture media. This secreted protein may be measured using the ToxReporter ELISA kit (Catalog # ETR-201K).

The ToxReporter Cell Line Starter Pack 2 (Catalog # ECLSP-002) includes ToxReporter AP-1 Stimulant (Catalog # ESTM-002), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately.

The ToxReporter Cell Line Starter Pack 5 (Catalog # ECLSP-005) includes ToxReporter NFĸB Stimulant (Catalog # ESTM-005), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately.

The ToxReporter Cell Line Starter Pack 3 (Catalog # ECLSP-003) includes ToxReporter GRE Stimulant (Catalog # ESTM-003), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter™ GRE Cell Line Starter Pack is required for use with the GRE ToxReporter Cell Line (Catalog #…

The ToxReporter Cell Line Starter Pack 1 (Catalog # ECLSP-001) includes ToxReporter p21 Stimulant (Catalog # ESTM-001), Selection Agent (Catalog # ESA-001), and Assay Media Supplement (Catalog # EAMS-001). Each component is sufficient for 100 assay points. If needed, each individual component may be purchased separately. ToxReporter™ p21 Cell Line Starter Pack is required for use with the p21 ToxReporter Cell Line (Catalog #…