Separations is a fundamental scientific discipline focused on the isolation and purification of components from mixtures. Through techniques such as chromatography, distillation, and filtration, separations scientists separate substances based on their physical or chemical properties. This field plays a crucial role in various industries, including pharmaceuticals, food and beverage testing, environmental science, materials research, and more. Explore how separations techniques enable precise analysis, quality control, and the development of innovative products.
The 1260 Infinity II Variable Wavelength Detector enables highest productivity with fast analysis at up to 120 Hz data rates. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.
The Agilent Protein 80 and Protein 230 kits provide a reliable way to assess protein concentration, identity, and purity. It eliminates handling of SDS-PAGE slab gels, staining or imaging steps.
These Gilson engineered caps produce a positive pressure seal with the needle in the Gilson ASPEC, ASPEC XL and ASPEC XL4 solid phase extraction systems.
Conversion of cartridge-based methods to an automation friendly 96-well format has never been easier or faster. The same trusted silica-based sorbents in Bond Elut cartridge products are now available in the streamlined Bond Elut 96-well plates. Bond Elut 96 components are specially formulated to offer superior cleanliness, flow reproducibility, and reliability.
Bond Elut 96 Square-well Plates are specially designed to offer superior cleanliness, flow reproducibility, and reliability, ensuring trouble-free, high throughput operation. Conversion of cartridge-based methods to an automation-friendly 96-well plate format has never been easier or faster.
Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic, and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from biofluids.
Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge.
Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The small particle size of the Bond Elut Alumina range ensures high extraction efficiency even when small bed masses are used.
Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive of all alkyl group bonded phases for nonpolar compounds. However, due to the extensive endcapping of this sorbent to mask polar silanol activity, retention, and elution of polar and multifunctional analytes can still be achieved.
Bond Elut EWP is based upon standard particles size silica but with 500 pores to allow more efficient extraction of large molecules (>15,000 MW), which are typically excluded from standard porosity silica phases.
Bond Elut C18 OH is a nonendcapped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
Bond Elut C2 is a fairly nonpolar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.
Bond Elut C8 is very similar in property to C18, but is not as retentive for nonpolar compounds due to its shorter hydrocarbon chain and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution.
Bond Elut Carbon cartridges are packed with ultrapure graphitized carbon particles that have been optimized for the absorption of pigments in food, fruits and vegetables, and small organic residues in wastewater. The powerful retention mechanisms of these products are appropriate for a broad range of analytes. In addition, careful manufacturing techniques result in lower carbon fines on the wall of the device.
CBA is a midpolarity sorbent and weak cation exchanger (pKa 4.8). It can be used with a wider range of counterions than lower pKa sorbents like SCX, and will demonstrate easier elution of quaternary amine functionalized analytes.
Bond Elut Cellulose columns utilize a pure microgranular cellulose powder that is packed between two, 20 µm polyethylene frits. The cellulose phase is very stable over a wide pH range and contains an extremely low metal content (Fe and CU content less than 5 ppm).
The Bond Elut Certify extraction cartridge utilizes a packed bed consisting of a special, nonpolar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extracting a wide range of compounds from a diverse number of aqueous matrices.
Bond Elut Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and strong anion-exchange (SAX) sorbent. It has been optimized for acidic drugs, such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen, and other compounds tha…
Bond Elut CH is a midpolarity sorbent that exhibits unique selectivities for certain analytes. When employed as a nonpolar sorbent, CH has the approximate polarity of a C2 sorbent. Bond Elut CH is often a good choice when nonpolar sorbents, such as C18, C8, or C2, do not provide the desired selectivity.
A medium polarity sorbent with many uses, Bond Elut Cyano (CN) is ideal for applications in which extremely nonpolar compounds would be irreversibly retained on high carbon load sorbents, such as C8 and C18. This endcapped version of the cyano sorbent is best utilized when extracting analytes from an aqueous matrix.
Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties, but with a slightly lower capacity as an anion-exchange sorbent. DEA has a moderately nonpolar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C8, but less polar than C2 or CN-E.
Bond Elut Diol resembles unbonded silica as it is known for strong hydrogen bonding with analytes. 20H can also be employed in the nonpolar mode because the hydrocarbon spacer on its functional group provides enough nonpolar character for retention of hydrophobic analytes. Bond Elut Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.
Bond Elut ENV, a PS-DVB polymer, is designed for the extraction of polar organic residues. It contains 125 µm spherical particles with a high degree of cross linking, advantageous for high volume, fast flowthrough applications.
Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar in nature and ideal for the isolation of polar compounds from nonpolar matrices. The larger particle size of the sorbent enables fast flow for large sample volumes and therefore can be an attractive alternative to silica if the sample matrix is particularly viscous.
Bond Elut LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics are crucial to allow compatibility with LC/MS or other delicate analytical techniques.
