Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
CHEMICON's NS225 Neurite Outgrowth Assay Kit (1 μm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsibl…
ApopTag® Equilibration Buffer is intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
Rat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
APOPTAG® Plastic Coverslips are designed to be custom fitted to the specific APOPTAG® kit application.
The ApopTag® Peroxidase In Situ Oligo Ligation Kit specifically detects apoptosis by staining only cells that contain double-stranded breaks that are blunt-ended or have a one base 3' overhang (cells containing nicked, gapped, 3'-recessed, 3'-overhanging ends longer than one base and single-stranded ends are not detected). The high analytic specificity of the ApopTag® ISOL Kit minimizes background due to non-specific labeling…
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
1X CHAPS Lysis Buffer is a component of the gel- and ELISA-based TRAPeze® Telomerase Detection Kits and is used in the extraction step of the procedure.
The TRAPEZE® XL Kit is a highly sensitive in vitro assay for the fluorometric detection of telomerase activity. As in the original TRAPEZE® Kit, primer sequence modifications that reduce amplification artifacts and an internal PCR control are included. In addition, the TRAPEZE® XL Kit uses fluorescence energy transfer (ET) primers to generate fluorescently labeled TRAP products which permit nonisotopic, quantitative analysis o…
The TRAPEZE® ELISA Kit provides the reagents necessary for performing the TRAP assay followed by ELISA detection of telomerase activity in cell/tissue samples.
The Amplifluor™ UniPrimer™-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3' single-stranded oligonucleotide tail and is provided at a concentration of 5.0 μM (25.0 mL).
The Amplifluorâ„¢ SNPs Genotyping System for Assay Development is designed for new users unfamiliar with the technology. This kit contains the fluorescein-, sulforhodamine- and JOE-labeled Amplifluorâ„¢ UniPrimersâ„¢ as well as control primers and templates.
The Amplifluorâ„¢ SNPs HT Genotyping System FAM-SR is designed for users familiar with Amplifluorâ„¢ and is for use with fluorescent plate readers.
The Amplifluorâ„¢ SNPs HT Genotyping System FAM-JOE is designed for users familiar with Amplifluorâ„¢ and is for users of real-time PCR detection instruments.
CHEMICON's Sure Blot® CHEMI™ Hybridization & Detection Kit is designed to provide high quality, non-radioactive detection of digoxigenin-labeled DNA probes hybridized to Sure Blot® Nylon Membrane. As an integrated hybridization and detection system, Sure Blot® CHEMI™ is quality controlled to produce high signal and low background. Comparable sensitivity to 32P is typically observed for single-copy gene detection. Sufficient re…
GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1 provided bound to glutathione agarose. Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates.
GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum.
For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3
A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity 90%.
Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog
This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the wel…
The cocktails in this kit are intended for the inhibition of cellular Serine/Threonine protein phosphatases, Tyrosine phosphastases, acid and alkaline phosphatases.
This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.
Accessory reagent kit for the analysis of histone methyltransferase activity.
