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Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.

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Human Amyloid β42 Brain ELISA

Merck

EMD Millipore’s Human Amyloid β42 Brain ELISA kit is used for the measurement of Amyloid beta 42 in brain samples and for other tissue samples in a 96-well format. This assay is for research use only and appropriate for the in vitro detection of human Amyloid β42 peptides in brain samples from e.g. Guinea pig, transgenic hAmyloid mice and cell extracts.

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Human Amylin ELISA

Merck

Amylin is a 37 amino acid hormone that is co-secreted with insulin from pancreatic beta cells in response to hyperglycemia.

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Human Adiponectin ELISA

Merck

Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.

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Human Total Proinsulin ELISA

Merck

Proinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures the intact and intermediate metabolic forms of human proinsulin.

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Human Pancreatic Polypeptide (PP) Elisa Kit

Merck

This Human Pancreatic Polypeptide (PP) ELISA kit is used for the non-radioactive quantification of Human PP in human serum, plasma, tissue extract and cell culture samples. One kit is sufficient to measure 39 unknown samples in duplicate

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Mouse Adiponectin ELISA

Merck

Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.

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Mouse SAA-3 ELISA

Merck

Serum Amyloid A3 (SAA-3) is an acute phase protein produced in the liver. In response to injury, granulocytes and macrophages secrete cytokines which modulate the production of acute phase proteins. Many of these proteins, such as SAA-3, α1-acid glycoprotein (AGP), lipoclain 24P3, plasminogen activator inhibitor-1 (PAI-1) and PTX3 are secreted from adipocytes and are elevated in various metabolic disease states.

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Rat Adiponectin ELISA

Merck

Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulation blood and influences systemic metabolism.

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Rat/Mouse Ghrelin (active) ELISA

Merck

Rat/Mouse Ghrelin (Active) ELISA kit for the quantification of active ghrelin in rat and mouse serum or plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.

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Rat Leptin ELISA

Merck

Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.

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Rat/Mouse C-Peptide 2 ELISA

Merck

EMD Millipore is pleased to offer the Rat/Mouse C-Peptide 2 ELISA kit for the quantification of C-peptide 2 in rat and mouse serum and plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.

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Rat/Mouse Growth Hormone ELISA

Merck

Growth Hormone (GH) is a 23 kDa circulating pituitary hormone, Rat GH shares approximately 97% homology with mouse GH and 65% homology with human GH in its protein sequence.

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Rat/Mouse GIP (total) ELISA

Merck

GIP, also known as gastric inhibitory polypeptide, or glucose-dependent insulinotropic polypeptide, is a 42 amino acid peptide hormone synthesized in and secreted from K cells in the intestinal epithelium.

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OxyELISA™ Oxidized Protein Quantitation Kit

Merck

The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see…

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TMB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (soluble)

Merck

TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum of 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing…

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Sterivac™ Filter Units

Merck

Sterivac vacuum-driven filter units are ideal for bottle-top filtering directly into receiver flasks at flow rates as fast as 1.5 L/min. The unique "start & stop" filtration action makes setting up, filtering and transferring the unit to another receiver fast and easy. Push a button to stop filtering, move the device to an empty bottle and restart

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Centricon Plus-70 Centrifugal Filter Units

Merck

• Concentrating and desaltingchromatography column eluates• Concentrating monoclonal antibodies• Concentrating proteins or viruses from culture supernatants• Clarifying tissue homogenates and cell lysates• Buffer exchange or diafiltration

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Montage® Antibody Purification Kits

Merck

Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…

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