Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
The GS Junior Titanium PicoTiterPlate Kit, combined with the GS Junior Titanium Sequencing Kit, provides the devices and reagents required for determining the nucleotide sequence of an immobilized and clonally amplified DNA library. The DNA-carrying beads and sequencing reagents are deposited in layers into the wells of a PicoTiterPlate (PTP) device, such that each well contains no more than a single DNA bead. The loaded PTP d…
The GS Junior Titanium Control Bead Kit can be used as a standard sequencing control on the GS Junior Instrument. During a test sequencing Run, only Control Beads are sequenced. The Control Beads contain known DNA sequences that should yield sequencing results within expected parameters if the instrument is performing to specifications. The GS Junior Titanium Control Bead Kit is used in combination with a GS Junior Titanium Se…
The GS Junior Titanium emPCR Kit (Lib-A) provides reagents for the amplification of an Amplicon library from a single effective (i.e., functionally clonal) bead-bound copy of the DNA template to tens of millions of copies per bead, amenable to sequencing with the GS Junior Instrument using the appropriate GS Junior Titanium kits. One of the main advantages of the emPCR Amplification process is that, while the entire procedure…
The GS Junior Titanium emPCR Kit (Lib-L) provides reagents for the amplification of a Shotgun, Paired End or cDNA library from a single effective (i.e., functionally clonal) bead-bound copy of the DNA template to tens of millions of copies per bead, amenable to sequencing with the GS Junior Instrument using the appropriate GS Junior Titanium kits. One of the main advantages of the emPCR Amplification process is that, while the…
The GS FLX Titanium emPCR Breaking Kit LV 12pc is used to break the emulsions and collect the beads from a single cup or up to a full day's worth of Large Volume Emulsion (LVE) Cups, providing they contain the same library. The kit contains components for 12 LV emulsion-breaking setups. Contents 12 pieces, each with one 8-channel transfer pipette, two 50 ml tube caps with tubing fittings, and tubing (approximately 1 meter in l…
Kit for preparing paired-end libraries of single-stranded DNA from double-stranded DNA input sample for subsequent amplification and sequencing on the Genome Sequencer FLX Instrument using the appropriate GS FLX Titanium Series kits. This kit contains the components for the protocols: adaptors, primers, and carrier DNA; other supplies and reagents are required to be purchased by the user as outlined in the manual. RESTRICTION…
The GS FLX Titanium emPCR Kits (Lib-A) contain all reagents and other components required for two large-volume (LV), medium-volume (MV) or small-volume (SV) emulsion-based clonal amplifications of DNA libraries prepared using the Genome Sequencer System library preparation procedure (Amplicons), for subsequent sequencing on a Genome Sequencer FLX Instrument using the appropriate GS FLX Titanium series Sequencing and PicoTiterP…
Kits for large-volume (LV), medium-volume (MV) and small-volume (SV) emulsion-based clonal amplification of a DNA library prepared using the appropriate GS FLX Titanium series library preparation procedure. The GS FLX Titanium emPCR Kits (Lib-L) contain all reagents and other components required for two emulsion-based clonal amplification reactions (which can be pooled, if applicable). Contents The GS FLX Titanium emPCR Kits…
The IEF100 is the only first dimension instrument that controls the current and voltage applied to the IPG strips to prevent overheating. It has flexible first-dimension IEF can be run with up to six 7 to 24 cm IPG strips simultaneously, or twelve 7 cm IPG strips using the included dual electrode accessory. The unit has turn and click intuitive user interface with graphical display, and is capable of faster run times with an i…
Combines the advantages of small format and efficient cooling for rapid screening of proteins and nucleic acids. The Hoefer™ SE260 small format vertical slab gel unit is intended for rapid electrophoresis of protein or nucleic acid samples. Most samples can be run in as little as 45 minutes, and only a minimal amount of sample is required.SE260 Deluxe Mighty Small Mini Vertical Unit Features: Maximum glass plate size 10 x…
High efficiency broad spectrum DNA transfection of cells in 3D culture for biologically relevant dataIdeal for the transfection of cells in 3D cell culture, the new 3D Transfection System combines the technologies of Reinnervate's alvetex® 3D Cell Culture Plates and Mirus Bio's TransIT®-3D Transfection Reagent, enabling scientists to create models that more accurately mimic the tissue environment, gaining a much deeper insight…
High efficiency electroporation using any instrumentIngenio® Electroporation Kits and Solution provide a universal, high efficiency, low toxicity solution for electroporation of DNA or siRNA into hard to transfect cell types. Electroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Electroporation Solution Features:•High eff…
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity. Ingenio Kits and Solution are compatible with multiple conventional electroporation instruments including Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser and Harvard-BTX® electroporators.Ingenio® Cuvettes Features:•High effi…
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Cell Droppers Features:•High efficiency electroporation of hard to transfect cell lines and primary cells•Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®•Idea…
High, reproducible protein yield in suspension CHO and 293 cellsTransIT-PRO® Transfection Kit consists of a DNA transfection reagent and boost combination specifically developed for mammalian protein production in suspension 293 and CHO derived cells.Reach critical stages faster. Decrease time to produce usable protein by maximizing target protein yields through transient transfection. The TransIT-PRO® Transfection Kit uses an…
A broad spectrum, low toxicity DNA transfection reagent optimized for 96-well plate applicationsTransIT®-Express Transfection Reagent is specifically developed for high-throughput transfection of plasmid DNA into mammalian cells. TransIT-Express Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and repro…
A broad spectrum, low toxicity, DNA transfection reagentTransIT®-LT1 Transfection Reagent is a broad spectrum reagent that provides high efficiency plasmid DNA delivery in many mammalian cell types including primary cells. TransIT-LT1 is a low toxicity, serum-compatible transfection reagent that eliminates the need for any culture medium change.TransIT-LT1 is suitable for both transient and stable transfection and can be used…
A high performance, animal-free, broad spectrum DNA transfection reagentTransIT®-2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT-2020 affords high levels of gene expression in typically hard-to-transfect cell types, including primary cells and is suitable for both transient and stable transfection.Tra…
A high efficiency DNA transfection reagent optimized for Jurkat and other hard-to-transfect cell typesTransIT®-Jurkat Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Jurkat cells and cell types of associated lineage.TransIT-Jurkat reagent also works well for additional cell lines of hematopoietic origin such as THP-1(human acute monocytic leukemia cell line) and R…
A high efficiency, low toxicity, DNA transfection reagent optimized for prostate cell typesTransIT®-Prostate Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Prostate cell types. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and reproducibil…
A high efficiency, low toxicity, DNA transfection reagent optimized for various neural cell typesTransIT-Neural® Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in various neural cell types. TransIT-Neural Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simpli…
A high efficiency, low toxicity, DNA transfection reagent optimized for CHO cell typesTransIT®-CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in CHO cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for 3T3 cell typesTransIT®-3T3 Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in NIH 3T3 cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of…
A high efficiency, low toxicity, DNA transfection reagent optimized for COS cell typesTransIT®-COS Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in COS cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for keratinocyte cellsTransIT®-Keratinocyte Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in keratinocyte cell types such as NIKS (Near-Diploid Immortalized Keratinocytes) and primary keratinocytes. TransIT-Keratinocyte Reagent provides all the attributes of the trusted TransIT series of transfecti…
