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Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.

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Fluorescence Microplate Assays

Thermo Fisher Scientific

Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.

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Single Cellome™ Unit SU10

Yokogawa Corp. of America

SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).

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Phytocannabinoid Neutrals Mixture 8 (CRM)

Cayman Chemical Co.

Phytocannabinoid Mixture 8 (CRM) (Item No. 34043) is a certified reference material mixture that contains neutral, non-acid, forms of compounds that can be isolated from plants in the genus Cannabis.  Included are Δ9-THC, Δ8-THC, cannabinol, cannabidiol, (±)-cannabichromene, cannabidivarin, cannabigerol, and tetrahydrocannabivarin. It is provided as a DEA exempt preparation in an amber ampule in which the headspace has been p…

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CRISPRi Libraries, Pools, and gRNAs

Merck

Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequence using modified dCas9 + gRNA complexes. Gene knockdown is complementary to CRISPR-KO and CRISPRa (activation) and has distinct advantages over existing loss-of-function strategies like RNAi.…

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