Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.
With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment.
The largest and most validated shRNA collection from the RNAi consortium (TRC). Merck's unique shRNA formats leverage the discovery potential of the trusted and proven TRC shRNA collection. When you partner with them, you gain access to their world class lentiviral production expertise and the formats and specifications for RNAi knockdown experiments at any scale.
This guideline describes the design, development, and validation of quantitative assays for proteins and peptides by mass spectrometry.
For transient gene repression with CRISPR-level specificity
Horizon's CRISPRi guide RNA in an easy to deliver lentiviral format
Horizon's proprietary dCas9-SALL1-SDS3 CRISPRi repressor in lentiviral particle format
A direct and efficient set of tools to study a gene’s function by transcriptional interference in its native context
Bioinformatics and Protein Array Services
Profiling against cancer antigens. Using the CTA Protein Array, Sengenics can quantify autoantibodies against more than 200 clinically relevant cancer testis antigens. These are tumor antigens normally expressed in germ cells but not in adult somatic tissues. In cancers, this regulation can be disrupted, resulting in CTA expression in tumors. The CTA array is ideal for research related to cancer immunotherapy.
Cancer drug screening and discovery. The OncoRex p53 Cancer Array provides a biologically relevant screening tool since all p53 variants are correctly folded and functional using the patented KREX ® protein folding technology. Using the array, Sengenics can screen your therapeutic antibodies and protein binding compounds against more than 100 p53 wildtype and mutant variants by measuring their impact on DNA binding as a func…
Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.
High-throughput online automated sample concentration LC-MS solution for water and beverage analysis
Automate complex and time-consuming sample preparation workflows integrated with LC-MS analysis
SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).
A high-performance RNA sequencing (RNA-Seq) kit that captures long and short RNAs in a single library, even from limited and low-quality samples. The kit provides all of the components required to make an RNA-Seq library for Illumina platforms. The size of the kit is for 24 reactions.
A high-performance RNA sequencing (RNA-Seq) kit that captures long and short RNAs in a single library, even from limited and low-quality samples. The kit provides all of the components required to make an RNA-Seq library for Illumina platforms. The size of the kit is for 96 reactions
For use with SEQuoia Complete Stranded RNA Library Prep Kits. Contains 12 unique dual indexed primers supplied in 12 separate vials, for 96 reactions
For use with SEQuoia Complete Stranded RNA Library Prep Kits. Contains 96 unique dual indexed primers plated in a 96-well plate, for 96 reactions
Use the SEQuoia RiboDepletion Kit to eliminate cytoplasmic ribosomal RNA (rRNA) and mitochondrial ribosomal RNA (mt rRNA) sequences from an RNA-Seq library.
25 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 25 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
100 x 20 µl reactions; kit includes 400 µl Reliance Select 5X reaction mix, 100 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
500 x 20 µl reactions; bundle of 5 boxes of 100 reaction kit; includes 2,000 µl Reliance Select 5X reaction mix, 500 µl Reliance reverse transcriptase, Reliance Select random primers, oligo(dT), DNase and buffer, nuclease-free water
Provides sensitive and specific quantitation of antibodies against four SARS-CoV-2 proteins: nucleocapsid, receptor binding domain, spike 1, and spike 2 viral proteins.
