Drug discovery > Target Discovery Products & Reviews

The Human CRISPR inhibition (CRISPRi) library (Dolcetto) is a single compact library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening using a KRAB-dCas9 effector.

Calculate drug toxicity and safety endpoints

MegaRobo, as the premier global provider of laboratory automation solutions, independently developed its sample reformatter and has received continuous praises from customers. The system integrates artificial intelligence and automated control for flexibility and for user-friendliness. The MRA-CDS-102 model can easily fit into biosafety cabinets with convenient installation.   

Megarobo, as the premier provider of laboratory automation solutions in China. CDS-610 sample reformatter, as one of the highest throughput of a single device on the market, integrates steps requiring extensive manual operation into a closed negative pressure system, to achieve a fully automated sample preparation "tube in and plate out".   

Megarobo's new MRA-LSF-840 series ultra-high-throughput kit assembly system can meet the needs of reagent card assembly in terms of throughput, compatibility, and yield. At the same time, it is equipped with card loading and packaging modules, and each module can be operated independently or combined for production.  

Efficient: Up to 8000-10000 pcs/h Flexible: Compatible with multiple types of tubes such as disposable virus sampling tubes Intuitive: Visualization software & touch screen Modular: Including laminar flow, labeling and coding, and CCD modules, allows for personalization Stable: Stable operation, high yield rate Effortless: Only 1 -2 people are needed to complete the whole process

Efficient: Up to 8000-10000 pcs/h Flexible: Compatible with multiple types of tubes such as disposable virus sampling tubes Intuitive: Visualization software & touch screen Modular: Including laminar flow, labeling and coding, and CCD modules, allows for personalization Stable: Stable operation, high yield rate Effortless: Only 1 -2 people are needed to complete the whole process

The Organoid Analysis Module for the CytoSMART Omni systems is an advanced machine-learning algorithm for robust organoid quantification, tracking, and analysis. 

The 10x Compatible Human CRISPRi Kinase Phosphatase Drug Targets Kit (Druggable Genome) contains one sub-pool of the top two ranked sgRNAs per gene for increased sensitivity and includes non-targeting controls built-in.

The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening.

Easily identify hits from pooled shrna screens

Design gRNA or search pre-designed guides

The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.

The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools.

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.

Developed at the Broad Institute, the MISSION ® TRC3 human whole genome pooled ORF library consists of over 17,000 unique ORF clones, encoding for over 14,000 genes used for overexpression screening

The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format

This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells

Utilize the power of CRISPR Whole Genome Lentiviral Pools to knockout and screen every gene in the human genome. The Sigma Whole Human Genome Lentiviral CRISPR Pool is provided as 200 ul in 8 x 25 ul aliquots at a minimum titer of 5x108 viral particles/ml via p24

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Recombinant Cas9-D10A Nickase protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments.

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Human CRISPRi Kinase Phosphatase Drug Targets Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in.