Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Exiqon miRCURY LNA™ microRNA Knockdown Probes High-affinity miRCURY LNA microRNA Knockdown Probesfor specific in vitro knockdown of microRNAs. Featuring minimal cytotoxicity and high biological stability. Key features of miRCURY LNA™ microRNA Knockdown Probes include:• Effective and sequence-specific antisense inhibition of microRNAs • Probes available for all known microRNAs • Superior biological stability • Minimal cytotoxi…
New Release 2009! 3D Prime Culture Medium. Improving still further our originial medium for 3D culture of epidermal keratinocytes, CELLnTEC has now released a new formulation, known as 3D Prime.Developed using our established signalling-based approach, this completely defined medium enables more rapid establishment of a fully stratified epidermis. Using this medium, models can now be ready for experimentation in just 14-18 day…
Fits plastic or glass media bottles with 45mm neck sizes. Cellulose acetate (CA) membranes provide fast flow rates and low protein binding and are good for filtering cell culture media. Angled hose connector simplifies vacuum line attachment. Individually packaged, sterile and certified nonpyrogenic. Each system has the membrane material and pore size printed on the unit and is color-coded by membrane type for easy produ…
CnT-27 is a progenitor cell targeted (PCT) liquid culture medium kit including both basal medium and separate supplements, developed for improved isolation and growth of mammary epithelium. CnT-27 is fully defined, serum and BPE-free formulation. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
Gibco Geltrex Matrix is a soluble form of reduced growth factor (RGF) basement membrane extract (BME) purified from murine Engelbreth-Holm-Swarm tumor
Optimized to achieve over 50% transfection efficiency without sacrificing cellular health, each kit includes an optimized protocol, Prostate Reagent and Prostate Boost Reagent in sufficient quantities to conduct up to 500 transfections in 35mm wells.Features include:• Reduces levels of cell damage when compared to liposmal-based transfection reagents • Transfection can be conducted in serum-containing media to save time and el…
R&D Fluorokine Receptor Detection Kits - Biotinylated ligands for estimating receptor densities R&D Systems now offers a Human Activin A Biotinylated Fluorokine Kit (Catalog # NFACTA). This flow cytometry kit is designed for the detection of Activin A receptor-expressing cells by flow cytometry. Activin A is a disulfide-linked homodimer belonging to the TGF-beta superfamily that signals through Type I/Type II receptor comple…
R&D Mouse Obesity Antibody Array - Proteome Profiler Array - For the parallel determination of the relative levels of selected mouse Adipokines. R&D Mouse Obesity Array (Catalog # ARY013) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary. R&D Mouse Obesity Antibody Array for the meas…
ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency. Suitable fo…
The Neurite Outgrowth HCS Reagent Kit product provides a more effective solution for quantifying neurite outgrowth by combining high-content screening (HCS)-quality fluorescence reagents with a validated, automation-compatible protocol. Neurite Outgrowth Reagent Kit Features: Neuronal-specific fluorescent reagent identifies neuronal cell bodies and neurites in a heterogeneous cell population without isolation steps Val…
LifeXtend™ conjugate stabilizer/diluent is a proprietary multi-component reagent system that stabilizes antibody-HRP conjugates both at 4°C and at ambient temperature. By using LifeXtend™ HRP stabilizer/diluent in antibody conjugation the HRP conjugate can be stabilized during use and stored as pre-diluted, ready-to-use reagents thus eliminating waste and improving consistency from experiment to experiment. LifeXtend™ shows…
SIRT1 belongs to the class III family of HDACs, which are NAD+ dependent enzymes. SIRT1 modulators have been shown to play a beneficial role in treating diseases such as cancer, diabetes, metabolic disease and inflammation, making them attractive targets for the development of new drugs. The new HTRF® SIRT1 assay, developed by Cisbio Bioassays using its patented HTRF technology, is a homogeneous method for directly measuring S…
Specially designed for use with the Cecil Adept HPLC, Q-Adept HPLC, Merit HPLC and IonQuest ion chromatography.
The Phospho-mTOR Activation Kit measures phosphorylation of mTOR, a kinase involved in the initiation of ribosome biogenesis and translation.
These stains are intense, highly photostable and match the output wavelengths of common fluorescence instrumentation. Effective image analysis in HCS cell-based assays and fluorescence microscopy requires fluorescent labeling of the entire cell. In these assays, the cellular primary object is used to identify and count individual cells and define the cell region in which the image analysis is applied. The primary object migh…
This kit enables simultaneous measurements in the same cell of six independent parameters that monitor cell health, including cell loss, nuclear size and morphological changes, mitochondrial membrane potential changes, cytochrome c release, and changes in cell permeability.
The BrdU and Ki67 Cell Proliferation HCS Reagent Kits are for simultaneous quantification of DNA replication and Ki67 proliferation marker in the same cell.
The Phospho-AKT Activation Kit measures phosphorylation of the 1, 2 and 3 isoforms of AKT, a key kinase involved in regulation of cell proliferation.
The Multiparameter Cell Death Detection Kits for high-content screening (HCS) enable simultaneous quantitation of LC3B protein on autophagic vesicles, cytochrome c localization and its release from mitochrondria, cell membrane integrity and its permeability and nuclear morphology and DNA content.
The micronucleus assay is an important component of genetic toxicology screening programs. Micronuclei arise from acentric chromosomes and lagging whole chromosomes.
The Phospho-JNK Detection Kit enables quantitation of phosphorylated Jun N-terminal kinase (phospho-JNK) expression in the nucleus.
The Autophagy & Proteasome Function: LC3B and Poly-Ubiquitin Detection Kits enable quantitation of LC3B protein on autophagic vesicles and ubiquitin on polyubiquitinated proteins.
The iNOS Activation Kits measure cytoplasmic induction of the iNOS enzyme via activation of one of the many inflammatory pathways.
The Cellomics Phospho-ATM Activation Kit contains optimized reagents for the detection and quantitation of phosphorylated ATM (Ser1981) in the nuclei.
The Mitotic Index HCS Reagent Kit provides high-content screening (HCS)-quality fluorescence reagents and a validated protocol for measurement of mitotic index, or the fraction of cells undergoing cell division within a population.
